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ACS Appl Mater Interfaces. 2017 May 24;9(20):16803-16812. doi: 10.1021/acsami.7b01555. Epub 2017 May 12.

Nanopillar Surface Topology Promotes Cardiomyocyte Differentiation through Cofilin-Mediated Cytoskeleton Rearrangement.

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Division of Cardiology, Department of Internal Medicine, Inje University College of Medicine, Seoul Paik Hospital , 9 Mareunnae-ro, Jung-gu, Seoul 04551, Republic of Korea.


Nanoscaled surface patterning is an emerging potential method of directing the fate of stem cells. We adopted nanoscaled pillar gradient patterned cell culture plates with three diameter gradients [280-360 (GP 280/360), 200-280 (GP 200/280), and 120-200 nm (GP 120/200)] and investigated their cell fate-modifying effect on multipotent fetal liver kinase 1-positive mesodermal precursor cells (Flk1+ MPCs) derived from embryonic stem cells. We observed increased cell proliferation and colony formation of the Flk1+ MPCs on the nanopattern plates. Interestingly, the 200-280 nm-sized (GP 200/280) pillar surface dramatically increased cardiomyocyte differentiation and expression of the early cardiac marker gene Mesp1. The gradient nanopattern surface-induced cardiomyocytes had cardiac sarcomeres with mature cardiac gene expression. We observed Vinculin and p-Cofilin-mediated cytoskeleton reorganization during this process. In summary, the gradient nanopattern surface with 200-280 nm-sized pillars enhanced cardiomyocyte differentiation in Flk1+ MPCs.


Flk1-positive mesodermal precursor cells; cardiomyocyte differentiation; cytoskeleton reorganization; gradient nanopattern plates; nanoimprinting

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