Format

Send to

Choose Destination
J Neuroimmunol. 2017 Jun 15;307:82-90. doi: 10.1016/j.jneuroim.2017.04.001. Epub 2017 Apr 7.

Expansion of IL-6+ Th17-like cells expressing TLRs correlates with microbial translocation and neurological disabilities in NMOSD patients.

Author information

1
Department of Microbiology and Parasitology, Federal University of the State of Rio de Janeiro, Brazil.
2
Departament of Genetics, Federal University of Rio de Janeiro, Brazil.
3
Neurology, Federal University of the State of Rio de Janeiro, Brazil.
4
Service de Neurologie A and Eugène Devic EDMUS Foundation against Multiple Sclerosis, Observatoire Français de la Sclérose en Plaques (OFSEP), Hôpital Neurologique Pierre Wertheimer, Hospices Civils de Lyon, 69677 Bron, France-Université Lyon 1, Université de Lyon, Lyon, France.
5
Department of Microbiology and Parasitology, Federal University of the State of Rio de Janeiro, Brazil; Neurology, Federal University of the State of Rio de Janeiro, Brazil. Electronic address: cbento@unirio.br.

Abstract

Different microbial antigens, by signaling through toll-like receptors (TLR), may contribute to Th17-mediated autoimmune diseases, such as neuromyelitis optica spectrum disorder (NMOSD). The objective of this study was to determine the proportion of different Th17-like cell subsets that express TLR in NMOSD patients. For this study, the frequency of different Th17 cell subsets expressing TLR subsets in healthy individuals (n=20) and NMOSD patients (n=20) was evaluated by cytometry. The peripheral levels of soluble CD14 (sCD14) and cytokines were determined by ELISA. Our results demonstrated that the proportion of peripheral CD4+ T cells expressing TLR2, 4 and 9 was significantly higher in NMOSD samples than in healthy subjects. In NMOSD, these cells are CD28+PD-1-CD57- and produce elevated levels of IL-17. Among different TLRs+ Th17-like subsets, the proportion of those that co-express IL-17 and IL-6 was significantly higher in NMOSD patients, which was positively correlated with sCD14 levels and EDSS score. By contrast, the percentage of TLRs+Treg17 cells (IL-10+IL-17+) was negatively related to sCD14 and the severity of NMOSD. In conclusion, the expansion of peripheral IL-6-producing TLR+ Th17-like cells in NMOSD patients was associated with both bacterial translocation and disease severity.

KEYWORDS:

CD14; NMOSD; Th17 phenotype; Toll-like receptor

PMID:
28495144
DOI:
10.1016/j.jneuroim.2017.04.001
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center