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RNA Biol. 2017 Jul 3;14(7):905-913. doi: 10.1080/15476286.2017.1325067. Epub 2017 May 11.

MicroRNA-338 modulates cortical neuronal placement and polarity.

Author information

1
a Department of Cognitive Neuroscience , Radboud University Medical Center , Nijmegen , The Netherlands.
2
d Donders Institute for Brain, Cognition, and Behaviour , Centre for Neuroscience , Nijmegen , The Netherlands.
3
b Department of Molecular Animal Physiology , Radboud University , Nijmegen , The Netherlands.
4
f Institute of Physiology, CAU Kiel University , Germany.
5
c Department of Human Genetics , Radboud University Medical Center , Nijmegen , The Netherlands.
6
e Laboratory of Molecular Biology, National Institute of Mental Health , National Institutes of Health , Bethesda , MD , USA.

Abstract

The precise spatial and temporal regulation of gene expression orchestrates the many intricate processes during brain development. In the present study we examined the role of the brain-enriched microRNA-338 (miR-338) during mouse cortical development. Reduction of miR-338 levels in the developing mouse cortex, using a sequence-specific miR-sponge, resulted in a loss of neuronal polarity in the cortical plate and significantly reduced the number of neurons within this cortical layer. Conversely, miR-338 overexpression in developing mouse cortex increased the number of neurons, which exhibited a multipolar morphology. All together, our results raise the possibility for a direct role for this non-coding RNA, which was recently associated with schizophrenia, in the regulation of cortical neuronal polarity and layer placement.

KEYWORDS:

Epigenetic gene regulation; in utero electroporation; neurodevelopment; neuronal migration; schizophrenia

PMID:
28494198
PMCID:
PMC5546544
DOI:
10.1080/15476286.2017.1325067
[Indexed for MEDLINE]
Free PMC Article

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