Atypical enteropathogenic Escherichia coli (aEPEC) in children under five years old with diarrhea in Quito (Ecuador)

Int Microbiol. 2016 Sep;19(3):157-160. doi: 10.2436/20.1501.01.273.

Abstract

Enteropathogenic Escherichia coli (EPEC) remain one the most important pathogens infecting children and they are one of the main causes of persistent diarrhea worldwide. In this study, we have isolated EPEC from 94 stool samples of children under five years old with diarrheal illness in the area of Quito (Ecuador), and we have determined the occurrence of the two subtypes of EPEC, typical EPEC (tEPEC) and atypical (aEPEC), by PCR amplification of the genes eae (attaching and effacing) and bfp (bundle- forming pilus). Typical EPEC is positive for eae and bfp genes while aEPEC is positive only for eae. Our results suggest that aEPEC is the most prevalent subtype in Quito (89.36 %), while subtype tEPEC is less prevalent (10.64 %). [Int Microbiol 19(3):157-160 (2016)].

Keywords: Escherichia coli; Quito (Ecuador); atypical EPEC; diarrhea in children; genes eae and bfp.

MeSH terms

  • Child, Preschool
  • Diarrhea / epidemiology
  • Diarrhea / microbiology*
  • Ecuador
  • Enteropathogenic Escherichia coli* / genetics
  • Escherichia coli Infections / epidemiology*
  • Escherichia coli Proteins / genetics
  • Humans
  • Infant

Substances

  • Escherichia coli Proteins