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APMIS. 2017 Jul;125(7):655-664. doi: 10.1111/apm.12695. Epub 2017 May 11.

High doses of recombinant mannan-binding lectin inhibit the binding of influenza A(H1N1)pdm09 virus with cells expressing DC-SIGN.

Author information

1
Division of Infection Disease, Zhejiang Key Laboratory for Neonatal Diseases, Children Hospital, Zhejiang University School of Medicine, Hangzhou, China.
2
Laboratory of Cancer Biology, Sir Runrun Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, China.
3
Department of Pediatrics, Pingxiang Maternal and Child Health Hospital, Pingxiang, China.

Abstract

The pandemic influenza A (H1N1)pdm09 virus continues to be a threat to human health. Low doses of mannan-binding lectin (MBL) (<1 μg/mL) were shown not to protect against influenza A(H1N1)pdm09 infection. However, the effect of high doses of MBL has not been investigated. Dendritic cell-specific intercellular adhesion molecule-3 grabbing non-integrin (DC-SIGN) has been proposed as an alternative receptor for influenza A(H1N1)pdm09 virus. In this study, we examined the expression of DC-SIGN on DCs as well as on acute monocytic leukemia cell line, THP-1. High doses of recombinant or human MBL inhibited binding of influenza A(H1N1)pdm09 to both these cell types in the presence of complement derived from bovine serum. Further, anti-DC-SIGN monoclonal antibody inhibited binding of influenza A(H1N1)pdm09 to both DC-SIGN-expressing DCs and THP-1 cells. This study demonstrates that high doses of MBL can inhibit binding of influenza A(H1N1)pdm09 virus to DC-SIGN-expressing cells in the presence of complement. Our results suggest that DC-SIGN may be an alternative receptor for influenza A(H1N1)pdm09 virus.

KEYWORDS:

Influenza A (H1N1)pdm09 virus; dendritic cell-specific intercellular adhesion molecule-3 grabbing non-integrin; mannan-binding lectin

PMID:
28493491
DOI:
10.1111/apm.12695
[Indexed for MEDLINE]

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