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Food Chem. 2017 Oct 1;232:10-18. doi: 10.1016/j.foodchem.2017.03.155. Epub 2017 Mar 30.

Effects of endogenous cysteine proteinases on structures of collagen fibres from dermis of sea cucumber (Stichopus japonicus).

Author information

1
School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China.
2
School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian 116034, PR China; Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL A1B3X9, Canada. Electronic address: zdyzf1@163.com.
3
School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian 116034, PR China.
4
School of Food Science and Technology, Dalian Polytechnic University, Dalian 116034, PR China; National Engineering Research Center of Seafood, Dalian 116034, PR China; Beijing Advanced Innovation Centre of Food Nutrition and Human Health, China Agricultural University, Beijing 100083, China; Tianjin Food Safety & Low Carbon Manufacturing Collaborative Innovation Center, Tianjin 300457, PR China. Electronic address: zhubeiwei@163.com.
5
Faculty of Fisheries Sciences, Hokkaido University, Hakodate, Hokkaido 041-8611, Japan.
6
Department of Biochemistry, Memorial University of Newfoundland, St. John's, NL A1B3X9, Canada.

Abstract

Autolysis of sea cucumber, caused by endogenous enzymes, leads to postharvest quality deterioration of sea cucumber. However, the effects of endogenous proteinases on structures of collagen fibres, the major biologically relevant substrates in the body wall of sea cucumber, are less clear. Collagen fibres were prepared from the dermis of sea cucumber (Stichopus japonicus), and the structural consequences of degradation of the collagen fibres caused by endogenous cysteine proteinases (ECP) from Stichopus japonicus were examined. Scanning electron microscopic images showed that ECP caused partial disaggregation of collagen fibres into collagen fibrils by disrupting interfibrillar proteoglycan bridges. Differential scanning calorimetry and Fourier transform infrared analysis revealed increased structural disorder of fibrillar collagen caused by ECP. SDS-PAGE and chemical analysis indicated that ECP can liberate glycosaminoglycan, hydroxyproline and collagen fragments from collagen fibres. Thus ECP can cause disintegration of collagen fibres by degrading interfibrillar proteoglycan bridges.

KEYWORDS:

Autolysis; Collagen fibres; Cysteine proteinases; Degradation; Interfibrillar proteoglycan bridges; Sea cucumber (Stichopus japonicus)

PMID:
28490052
DOI:
10.1016/j.foodchem.2017.03.155
[Indexed for MEDLINE]

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