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Cell Physiol Biochem. 2017;42(1):13-21. doi: 10.1159/000477107. Epub 2017 May 10.

Long Noncoding RNA CRNDE Promotes Proliferation of Gastric Cancer Cells by Targeting miR-145.

Author information

1
Department of General Surgery, Huashan Hospital, Fudan University, Shanghai, China.
2
Department of General Surgery, Shanghai Children's Medical Center, Shanghai, China.

Abstract

BACKGROUND/AIMS:

The colorectal neoplasia differentially expressed (CRNDE) gene is a long noncoding RNA (lncRNAs) that is upregulated in colorectal cancer and glioma. Here, we investigated the regulatory function of CRNDE in gastric cancer (GC).

METHODS:

CRNDE and miR-145 expression were assayed by qRT-PCR, and E2F3 protein expression was measured by western blotting. A luciferase reporter assay was used to detect the direct regulation of miR-145 by CRNDE. Cell viability and colony formation of human GC cells were detected using MTT and colony formation assay, respectively.

RESULTS:

CRNDE was highly expressed in GC cell lines and tissues; overexpression of CRNDE increased GC cell viability and promoted colony formation. Knockdown of CRNDE did not result in loss of expression-related effects on cell proliferation and colony formation. Further investigation revealed that the miR-145 target gene E2F3 was strongly expressed following CRNDE competitive molecular sponging of miR-145.

CONCLUSION:

CRNDE acted as a growth-promoting lncRNA in GC and maybe a potential target of GC treatment.

KEYWORDS:

CRNDE; E2F3; Gastric cancer; LncRNA; Proliferation; miR-145

PMID:
28490034
DOI:
10.1159/000477107
[Indexed for MEDLINE]
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