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Biosci Biotechnol Biochem. 2017 Jun;81(6):1241-1245. doi: 10.1080/09168451.2017.1282809. Epub 2017 Feb 28.

Effect of epigallocatechin-3-gallate on the increase in type II collagen accumulation in cartilage-like MSC sheets.

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a Division of Biotechnology and Macromolecular Chemistry , Graduate School of Engineering, Hokkaido University , Sapporo , Japan.
b School of Health and Sports Sciences , Mukogawa Women's University , Nishinomiya , Japan.
c Foundation for Biomedical Research and Innovation, International Medical Device Alliance , Kobe , Japan.


With the aim to increase type II collagen content in the scaffold-free cartilage-like cell sheet using human bone marrow mesenchymal stem cells, we examined the effect of epigallocatechin-3-gallate (EGCG) addition to the chondrogenic medium for the cell sheet culture. The addition of EGCG (10 μM) increased the content of type II collagen 2-fold, while the addition did not markedly change the expression level of the genes encoding type II collagen and Sox 9. The reactive oxygen species level in the cells in cell sheets was thought to be too low to suppress the accumulation of type II collagen. On the other hand, the addition of EGCG markedly decreased both the matrix metalloproteinase-13 concentration in the supernatant of cell sheet culture and the type II collagen degradation activity in that supernatant. Taken together, EGCG may enhance the accumulation of type II collagen by suppressing type II collagen degradation.


MMP-13; cartilage-like cell sheet; epigallocatechin-3-gallate; mesenchymal stem cell; type II collagen

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