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Biochem J. 1988 Oct 1;255(1):85-9.

Undifferentiated F9 embryonal carcinoma cells produce a short-chain collagen molecule.

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1
Collagen Research Laboratory, Veterans Administration Outpatient Clinic, Boston, MA 02108.

Abstract

The undifferentiated F9 embryonal carcinoma cells produce a unique collagen that decreases in amount during retinoic acid-induced differentiation of F9 cells into basement-membrane parietal endoderm. A bacterial-collagenase-sensitive protein of approx. 60,000 Da was resolved on polyacrylamide-gel electrophoresis. After pepsin digestion, two pepsin-resistant fragments containing hydroxyproline were demonstrated, suggesting that a portion of the molecule has a stable triple helix. The mRNA from the undifferentiated F9 cells translates a collagenase-sensitive protein with a molecular mass consistent with the 60,000 Da collagenous protein produced by undifferentiated F8 cells.

PMID:
2848513
PMCID:
PMC1135193
[Indexed for MEDLINE]
Free PMC Article
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