Format

Send to

Choose Destination
Neural Dev. 2017 May 8;12(1):7. doi: 10.1186/s13064-017-0085-2.

Afadin controls cell polarization and mitotic spindle orientation in developing cortical radial glia.

Author information

1
Department of Pathology, University of Illinois at Chicago, 909 S Wolcott Avenue, Chicago, IL, 60612, USA. jrakoto@uic.edu.
2
Department of Physiology, University of California, San Francisco, 1550 4th street, San Francisco, CA, 94158-2611, USA.
3
Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Dr Bohr Gasse 3-5, 1030, Vienna, Austria.
4
Department of Pathology, University of California, San Francisco, 513 Parnassus Avenue, San Francisco, CA, 94143-0502, USA.
5
Department of Physiology, University of California, San Francisco, 1550 4th street, San Francisco, CA, 94158-2611, USA. lreichardt@simonsfoundation.org.
6
Department of Pathology, University of Illinois at Chicago, 909 S Wolcott Avenue, Chicago, IL, 60612, USA.

Abstract

BACKGROUND:

In developing tissues, cell polarity and tissue architecture play essential roles in the regulation of proliferation and differentiation. During cerebral cortical development, adherens junctions link highly polarized radial glial cells in a neurogenic niche that controls their behavior. How adherens junctions regulate radial glial cell polarity and/or differentiation in mammalian cortical development is poorly understood.

RESULTS:

Conditional deletion of Afadin, a protein required for formation and maintenance of epithelial tissues, leads to abnormalities in radial glial cell polarity and subsequent loss of adherens junctions. We observed increased numbers of obliquely-oriented progenitor cell divisions, increased exit from the ventricular zone neuroepithelium, and increased production of intermediate progenitors.

CONCLUSIONS:

Together, these findings indicate that Afadin plays an essential role in regulating apical-basal polarity and adherens junction integrity of radial glial cells, and suggest that epithelial architecture plays an important role in radial glial identity by regulating mitotic orientation and preventing premature exit from the neurogenic niche.

KEYWORDS:

Adherens junctions; Apicobasal polarity; Cell fate; Cortical development; Primary cilia

PMID:
28482867
PMCID:
PMC5422985
DOI:
10.1186/s13064-017-0085-2
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for BioMed Central Icon for PubMed Central
Loading ...
Support Center