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Biomaterials. 2017 Aug;135:30-41. doi: 10.1016/j.biomaterials.2017.04.047. Epub 2017 Apr 28.

Control of angiogenesis and host response by modulating the cell adhesion properties of an Elastin-Like Recombinamer-based hydrogel.

Author information

1
Department of Surgery, University Hospital Basel, Switzerland; Department of Biomedicine, University Basel, Switzerland.
2
G.I.R. BIOFORGE, Universidad de Valladolid, Valladolid, Spain; Technical Proteins NanoBiotechnology S.L., Valladolid, Spain.
3
G.I.R. BIOFORGE, Universidad de Valladolid, Valladolid, Spain.
4
Institute of Pathology, University Hospital Basel, University of Basel, Switzerland.
5
G.I.R. BIOFORGE, Universidad de Valladolid, Valladolid, Spain. Electronic address: roca@bioforge.uva.es.
6
Department of Surgery, University Hospital Basel, Switzerland; Department of Biomedicine, University Basel, Switzerland. Electronic address: Anna.Marsano@usb.ch.

Abstract

The control of the in vivo vascularization of engineered tissue substitutes is essential in order to obtain either a rapid induction or a complete inhibition of the process (e.g. in muscles and hyaline-cartilage, respectively). Among the several polymers available, Elastin-Like Recombinamers (ELR)-based hydrogel stands out as a promising material for tissue engineering thanks to its viscoelastic properties, non-toxicity, and non-immunogenicity. In this study, we hypothesized that varying the cell adhesion properties of ELR-hydrogels could modulate the high angiogenic potential of adipose tissue-derived stromal vascular fraction (SVF) cells, predominantly composed of endothelial/mural and mesenchymal cells. Human SVF cells, embedded in RGD-REDV-bioactivated or unmodified ELR-hydrogels, were implanted in rat subcutaneous pockets either immediately or upon 5-day-culture in perfusion-bioreactors. Perfusion-based culture enhanced the endothelial cell cord-like-organization and the release of pro-angiogenic factors in functionalized constructs. While in vivo vascularization and host cell infiltration within the bioactivated gels were highly enhanced, the two processes were strongly inhibited in non-functionalized SVF-based hydrogels up to 28 days. ELR-based hydrogels showed a great potential to determine the successful integration of engineered substitutes thanks to their capacity to finely control the angiogenic/inflammation process at the recipient site, even in presence of SVF cells.

KEYWORDS:

Angiogenesis; Elastin-Like Recombinamers; Functionalization; Host reaction; Stromal vascular fraction

[Indexed for MEDLINE]

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