Send to

Choose Destination
See comment in PubMed Commons below
J Clin Endocrinol Metab. 2017 Aug 1;102(8):2751-2761. doi: 10.1210/jc.2016-3997.

Molecular Biomarkers for Weight Control in Obese Individuals Subjected to a Multiphase Dietary Intervention.

Author information

Institut National de la Santé et de la Recherche Médicale, Obesity Research Laboratory, Institute of Metabolic and Cardiovascular Diseases, 31432 Toulouse, France.
University of Toulouse, Paul Sabatier University, 31400 Toulouse, France.
Toulouse University Hospitals, Departments of Endocrinology, Metabolism and Nutrition, 31400 Toulouse, France.
Nestlé Institute of Health Sciences SA, CH-1015 Lausanne, Switzerland.
Department of Nutrition, Exercise and Sports, Faculty of Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
Department of Human Biology, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University Medical Centre, 6229 Maastricht, The Netherlands.
Unité de Mathématiques et Informatique Appliquées de Toulouse, Université de Toulouse, 31326 Castanet Tolosan, France.



Although calorie restriction has proven beneficial for weight loss, long-term weight control is variable between individuals.


To identify biomarkers of successful weight control during a dietary intervention (DI).

Design, Setting, and Participants:

Adipose tissue (AT) transcriptomes were compared between 21 obese individuals who either maintained weight loss or regained weight during the DI. Results were validated on 310 individuals from the same study using quantitative reverse transcription polymerase chain reaction and protein levels of potential circulating biomarkers measured by enzyme-linked immunosorbent assay.


Individuals underwent 8 weeks of low-calorie diet, then 6 months of ad libitum diet.

Outcome Measure:

Weight changes at the end of the DI.


We evaluated six genes that had altered expression during DI, encode secreted proteins, and have not previously been implicated in weight control (EGFL6, FSTL3, CRYAB, TNMD, SPARC, IGFBP3), as well as genes for which baseline expression differed between those with good and poor weight control (ASPN, USP53). Changes in plasma concentrations of EGFL6, FSTL3, and CRYAB mirrored AT messenger RNA expression; all decreased during DI in individuals with good weight control. ASPN and USP53 had higher baseline expression in individuals who went on to have good weight control. Expression quantitative trait loci analysis found polymorphisms associated with expression levels of USP53 in AT. A regulatory network was identified in which transforming growth factor β1 (TGF-β1) was responsible for downregulation of certain genes during DI in good controllers. Interestingly, ASPN is a TGF-β1 inhibitor.


We found circulating biomarkers associated with weight control that could influence weight management strategies and genes that may be prognostic for successful weight control.

[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Silverchair Information Systems
    Loading ...
    Support Center