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Fetal Pediatr Pathol. 2017 Aug;36(4):282-293. doi: 10.1080/15513815.2017.1315198. Epub 2017 May 8.

Fetal Cardiac Impairment in Nitrofen-Induced Congenital Diaphragmatic Hernia: Postmortem Microcomputed Tomography Imaging Study.

Author information

1
a Children's Hospital, Istituto Mediterraneo di Eccellenza Pediatrica, Pediatric Surgery Unit , Palermo , Italy.
2
b Pediatric Unit, University of Pavia and Fondazione IRCCS Policlinico San Matteo , Pavia , Italy.
3
c Department of Radiology , Studio Diagnostico Eco , Vimercate, Milan , Italy.
4
d University of Trieste, Institute of Pathologic Anatomy , Trieste , Italy.
5
e School of Medicine and Surgery, University of Milano , Milan , Italy.
6
f Fondazione IRCCS Policlinico San Matteo, Scientific Direction, Biometry & Clinical Epidemiology , Pavia , Italy.
7
g Department of Radiology , Cattinara Hospital , Trieste , Italy.
8
h Hôpitaux Pédiatriques de Nice CHU-Lenval , Nice , France.

Abstract

INTRODUCTION:

We assessed the post-mortem micro-CT utility to evaluate fetal cardiac impairment in nitrofen-induced congenital diaphragmatic hernia (CDH).

METHODS:

At 9.5d postconception (dpc), pregnant rats were exposed to nitrofen. At +18 and +21dpc, fetuses were harvested by cesarean section. Postmortem micro-CT and autopsy were performed. Fetuses were assigned to three experimental groups: Control group (C), Nitrofen group (N, exposition to nitrofen without CDH), CDH group. Cardio-pulmonary indices were evaluated.

RESULTS:

An accurate morphological evaluation of the lung and heart was obtained. Early cardiac impairment was present in the N and CDH groups. At term pregnancy, an increased maximum diameter and decreased minimum diameter of the ventricles and increased interventricular septal thickness were noted in CDH. Histology showed a myocardial "disarray" and an high density of mitotic myocytes in CDH at midgestation.

CONCLUSIONS:

The potential utility of post-mortem fetal micro-CT examination in CDH was introduced. The results highlighted the presence of cardiac adaptation in affected fetuses.

KEYWORDS:

Congenital diaphragmatic hernia; fetal cardiac impairment; microcomputed tomography; nitrofen; virtual autopsy

PMID:
28481127
DOI:
10.1080/15513815.2017.1315198
[Indexed for MEDLINE]

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