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Nat Commun. 2017 May 8;8:15150. doi: 10.1038/ncomms15150.

Antibody-powered nucleic acid release using a DNA-based nanomachine.

Author information

1
Department of Chemistry, University of Rome, Tor Vergata, Via della Ricerca Scientifica, 00133 Rome, Italy.
2
Laboratory of Biosensors and Nanomachines, Département de Chimie, Départment de Biochimie et Médecine Moléculaire, Université de Montréal, Montréal, Quèbec, Canada H3T 1J4.

Abstract

A wide range of molecular devices with nanoscale dimensions have been recently designed to perform a variety of functions in response to specific molecular inputs. Only limited examples, however, utilize antibodies as regulatory inputs. In response to this, here we report the rational design of a modular DNA-based nanomachine that can reversibly load and release a molecular cargo on binding to a specific antibody. We show here that, by using three different antigens (including one relevant to HIV), it is possible to design different DNA nanomachines regulated by their targeting antibody in a rapid, versatile and highly specific manner. The antibody-powered DNA nanomachines we have developed here may thus be useful in applications like controlled drug-release, point-of-care diagnostics and in vivo imaging.

PMID:
28480878
PMCID:
PMC5424144
DOI:
10.1038/ncomms15150
[Indexed for MEDLINE]
Free PMC Article

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