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Afr J Tradit Complement Altern Med. 2016 Nov 23;14(1):263-271. doi: 10.21010/ajtcam.v14i1.28. eCollection 2017.

INHIBITORY ACTIVITY OF MANGIFERIN ON HELICOBACTER PYLORI-INDUCED INFLAMMATION IN HUMAN GASTRIC CARCINOMA AGS CELLS.

Author information

1
Department of Oncology, Jining No. 1 People's Hospital, Shandong 272011, China.
2
Department of oncology, Qingdao Municipal Hospital, Shandong, 266000, China.

Abstract

BACKGROUND:

Gastric cancer is a serious health issue caused by H. pylori and claims more lives in developing and undeveloped countries. Hence, the need for a natural drug with several pharmacological activities with no adverse effect are highly recommended. The target of this study was to verify the anti-H. pyloric efficacy of mangiferin (MF) on H. pylori-infected AGS cells.

MATERIALS AND METHODS:

AGS cells were co-cultured with H. pylori and incubated with increased concentration of MF (10, 20, 50 and 100 μg/mL) or amoxicillin (AMX) and DMSO (control) group to assess its anti-H. pyloric effect by checking inhibitory zone, bacterial drug sensitivity test (MIC and MBC), adhesion and invasive property and various inflammatory markers.

RESULTS:

Co-culturing of H. pylori-infected AGS cells with MF (100 μg) considerably increased (p<0.05) the inhibitory zone as well as substantially lowered (p<0.05) in the levels of MBC and MIC with decreased adhesion and invasive property in a dose-dependent manner and thus endorsing its anti H. pyloric activity and are almost equivalent to antibiotic AMX. Meanwhile, inflammatory markers such as NF-κΒ subunit p65, interleukins-1β, IL-8, and TNF-α were also markedly suppressed (p<0.01) on treatment with MF. In addition, the protein expression of inflammatory enzymes like COX-2 and iNOS were notably downregulated (p<0.05) in AGS cells incubated with MF.

CONCLUSION:

We, concluded that MF treatment with H. pylori-infected AGS cells significantly suppressed the adhesion and invasion process as well as deactivated NF-p65 thereby blocking inflammatory response and thus lower the incidence of gastric carcinoma.

KEYWORDS:

AGS cells; Gastric cancer; H. pylori; amoxicillin; anti-inflammatory; mangiferin

PMID:
28480404
PMCID:
PMC5411878
DOI:
10.21010/ajtcam.v14i1.28
[Indexed for MEDLINE]
Free PMC Article

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