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Afr J Tradit Complement Altern Med. 2016 Nov 23;14(1):179-186. doi: 10.21010/ajtcam.v14i1.20. eCollection 2017.

IMMUNOSUPPRESIVE EFFECTS OF THE METHANOLIC EXTRACT OF CHRYSOPHYLLUM CAINITO LEAVES ON MACROPHAGE FUNCTIONS.

Author information

1
Laboratorio de Farmacología, Facultad de Química, Universidad Autónoma de Yucatán.
2
Laboratorio de Química Farmacéutica, Facultad de Química, Universidad Autónoma de, Yucatán.
3
Laboratorio de Bioquímica y Genética Molecular, Facultad de Química, Universidad, Autónoma de Yucatán.
4
Laboratorio de Cromatografía, Facultad de Química, Universidad Autónoma de Yucatán.
5
Centro de Información de Medicamentos, Facultad de Química, Universidad Autónoma de Yucatán.

Abstract

BACKGROUND:

The aim of this work was to evaluate the immunomodulatory effect of the methanol extract (MeOH) from Chrysophyllum cainito leaves on the MΦs functions.

MATERIAL AND METHODS:

Peritoneal murine MΦs isolated from Balb/c mice were treated with the MeOH extract and stimulated with LPS. The effect on the phagocytosis was evaluated by flow cytometry assay. The nitric oxide (NO) and hydrogen peroxide (H2O2) production was measured by the Griess reagent and phenol red reaction, respectively. Levels of IL-6 and TNF-α was measured using an ELISA kit. Viability of MΦs and Vero cells was determined by the MTT method.

RESULTS:

The MeOH extract of C. cainito leaves inhibited significantly the phagocytosis, and decreased IL-6 and TNF-α production as well as NO and H2O2 released by the MΦs, in a concentration-dependent manner. In addition, MeOH extract of C. cainito showed low cytotoxicity effect against the cells.

CONCLUSION:

These results suggest that MeOH extract of C. cainito leaves has an immunosuppressive effect on murine MΦs, without effects on cell viability. GC-MS chromatogram analysis of MeOH extract showed that lupeol acetate and alpha-amyrin acetate are the principal compounds.

KEYWORDS:

Chrysophyllum cainito; Immunomodulation; Macrophages; Phagocytosis; Sapotaceae

PMID:
28480396
PMCID:
PMC5411870
DOI:
10.21010/ajtcam.v14i1.20
[Indexed for MEDLINE]
Free PMC Article

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