Format

Send to

Choose Destination
EMBO J. 1988 Aug;7(8):2321-7.

Involvement of cdc13+ in mitotic control in Schizosaccharomyces pombe: possible interaction of the gene product with microtubules.

Author information

1
Cold Spring Harbor Laboratory, NY 11724.

Abstract

Previous genetic studies have shown that the fission yeast cdc13+ gene product interacts closely with the cdc2+ protein kinase during mitosis. Here, we have cloned the cdc13+ gene from a S. pombe gene bank by complementation of the temperature-sensitive defect of a cdc13-117 mutant strain. The complementing activity was localized to a 1.9-kb XbaI-NsiI DNA fragment, and nucleotide sequencing revealed a 1446-bp open reading frame. The predicted amino acid sequence contained 482 residues and was not homologous to any protein in a protein database. The cdc13+ gene function was confirmed to be essential for cell division since cells carrying a cdc13 null allele arrested with a cdc phenotype. However, unlike any existing temperature-sensitive cdc13 mutants, cdc13 null mutants arrested in G2 without septa or condensed chromosomes indicating that cdc13+ gene function is required at or prior to the initiation of mitotis. cdc13-117 mutant strains were found to be hypersensitive to the tubulin inhibitor thiabendazole. This observation suggests that the cdc13+ gene product, which is required for mitotic initiation, may interact with microtubules.

PMID:
2847913
PMCID:
PMC457096
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center