Format

Send to

Choose Destination
Chem Biol Interact. 2017 Jun 25;272:10-20. doi: 10.1016/j.cbi.2017.04.002. Epub 2017 May 4.

In vitro and in vivo anticancer activity of posterior salivary gland toxin from the cuttlefish Sepia pharaonis, Ehrenberg (1831).

Author information

1
Department of Medical Biotechnology, Chettinad Academy of Research and Education, Kelambakkam, 603 103, Tamil Nadu, India.
2
Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Kelambakkam, 603 103, Tamil Nadu, India.
3
Department of Pathology, Chettinad Hospital and Research Institute, Kelambakkam, 603 103, Tamil Nadu, India.
4
Faculty of Allied Health Sciences, Chettinad Academy of Research and Education, Kelambakkam, 603 103, Tamil Nadu, India. Electronic address: saranprp@gmail.com.

Abstract

Posterior salivary gland (PSG) toxins are high molecular weight toxins secreted by cephalopods and gastropods which possess immense potentials in biomedical applications. In the present study, the biomedical potentials of the PSG toxin from the cuttlefish, S. pharaonis was determined in vitro and in vivo. The cytostatic potentials of the PSG toxin was determined by the lymphocyte migration inhibition assay. The PSG toxin (50 μg/ml) effectively inhibited the migration of lymphocytes across the agarose gel matrix under the presence of lipopolysaccharide mitogen. The cytotoxicity of the PSG toxin against cancer cell lines was determined using the MTT assay. The PSG toxin exhibited highest cytotoxicity against the MCF-7 breast cancer cells (IC50-10.64 μM) followed by KB, HeLa and A549 cells. The PSG toxin also exhibited proportional release of LDH leakage by mitochondrial damage with an IC50-13.85 μM against MCF-7 breast cancer cells. Flow cytometry analysis revealed that the PSG toxin induced apoptosis in MCF-7 cells by cell cycle arrest at G0/G1 phase. The PSG toxin (80 mg/kg b.w.) exhibited pronounced reduction (29%) in tumor growth in experimentally induced breast carcinoma in female Balb/C mice, in vivo. Hematological analysis illustrated the restoration of blood and biochemical parameters by the PSG toxin in mice induced with tumor. Histopathology studies also revealed the restitution of morphological features in the mammary tumor and vital organs in mice treated with the PSG toxin without any observed toxicity and adverse effects. The PSG toxin further exhibited commendable potentials in the prevention of tumor metastasis into immediate organs viz lungs, thus functioning as an anti-metastatic agent. The results of the present study showed that the PSG toxin exhibited immense promise as a potential peptide based anticancer agent, in future.

KEYWORDS:

Anti-proliferative; Anticancer; Breast cancer; Flow cytometry; PSG toxin; S. pharaonis

PMID:
28477960
DOI:
10.1016/j.cbi.2017.04.002
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center