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Microcirculation. 2017 Aug;24(6). doi: 10.1111/micc.12379.

The impact of endotoxin on jejunal tissue oxygenation.

Author information

1
Department of Anesthesiology and Critical Care Medicine, Innsbruck Medical University, Innsbruck, Austria.
2
Institute of Anesthesiology and Critical Care Medicine II, Klinikum Wels, Wels, Austria.
3
Department for Medical Statistics, Informatics and Health Economics, Innsbruck Medical University, Innsbruck, Austria.
4
Department of Anesthesiology and Critical Care Medicine, Krankenhaus St. Vinzenz in Zams, Zams, Austria.

Abstract

OBJECTIVE:

We examined the effects of systemic ETX on jejunal mucoal microcirculatory parameters in anesthetized pigs.

METHODS:

Jejunal mucosal tissue PO2 was measured employing Clark-type surface oxygen electrodes. Oxygen saturation of jejunal microvascular hemoglobin was determined by tissue reflectance spectrophotometry. Jejunal microcirculatory blood flow was assessed by laser Doppler flowmetry. Microvascular conductance and rhythmical oscillation of the tissue PO2 were calculated. Systemic hemodynamic variables, mesenteric venous and systemic acid base and blood gas variables, and lactate measurements were recorded. Measurements were taken at BL and after Escherichia coli LPS administration in 20 minutes intervals for 110 minutes.

RESULTS:

ETX infusion led to a significant (P<.05) decrease of PO2 muc (from 24±4 to 8±4 mm Hg) and microvascular HbO2 (from 41±13 to 24±12%). Microcirculatory conductivity increased in ETX animals, microvascular blood flow remained unchanged (PU; from 228±45 to 232±58). ETX induced an increase in oscillation frequency of mucosal tissue oxygenation.

CONCLUSIONS:

Endotoxinemia resulted in a significant depression of mucosal tissue oxygenation despite a constant microcirculatory blood flow. This impairment of tissue oxygenation resulted in an increase in the vasomotion pattern in a futile attempt to counteract the undersupply of oxygen to the jejunal tissue.

KEYWORDS:

clark-type surface oxygen electrodes; microcirculatory flowmotion; mucosal oxygen tension; sepsis; vasomotion

PMID:
28477419
DOI:
10.1111/micc.12379
[Indexed for MEDLINE]

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