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Bratisl Lek Listy. 2017;118(4):223-227. doi: 10.4149/BLL_2017_044.

Effect of sodium aescinate treatment on PCOS rat model with insulin resistance.

Abstract

BACKGROUND:

Recent studies indicated that insulin resistance may contribute to the pathogenesis of polycystic ovary syndrome (PCOS); however, the specific mechanism is still unclear.

OBJECTIVE:

To investigate the effect of sodium aescinate (SA) on PCOS-IR rat models.

METHODS:

Sixty rats were randomly divided into the five groups: un-treated rats (n = 12), PCOS-IR group (n = 12), PCOS-IR group plus 50 mg/kg SA (n = 12), PCOS-IR group plus 10 mg/kg SA (n = 12), PCOS-IR group plus 150 mg/kg metformin (n = 12). On day 21, rats were sacrificed, and H(and)E staining was performed for histopathologic examination of the ovaries; moreover, the serum level of follicle-stimulating hormone (FSH), testosterone, and luteotropic hormone (LH) were measured, and the expression as well as phosphorylation of PI3K, Akt and Gsk-3β were examined using western blot assay.

RESULTS:

High dosage of SA treatment improved the morphological features of the ovaries in PCOS rats, and also induced significant decrease in serum expression of testosterone and LH/FSH ratio and significant decrease in the expression of p-PI3K, p-Akt and p-Gsk-3β.

CONCLUSION:

Our results demonstrated that SA treatment could alleviate the symptom of PCOS in rat model through regulating the PI3K/Akt/GSK3-β pathway (Fig. 4, Ref. 22).

KEYWORDS:

AKT GSK3-β.; PI3K; insulin resistance; polycystic ovary syndrome; sodium aescinate

PMID:
28471233
DOI:
10.4149/BLL_2017_044
[Indexed for MEDLINE]

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