Format

Send to

Choose Destination
Methods Mol Biol. 2017;1586:325-334. doi: 10.1007/978-1-4939-6887-9_21.

A Generic Protocol for Intracellular Expression of Recombinant Proteins in Bacillus subtilis.

Author information

1
VNUHCM-University of Science, 227 Nguyen Van Cu, District 5, Hochiminh City, Vietnam.
2
VNUHCM-University of Science, 227 Nguyen Van Cu, District 5, Hochiminh City, Vietnam. ndhoang@hcmus.edu.vn.

Abstract

Bacillus subtilis (B. subtilis) is a potential and attractive host for the production of recombinant proteins. Different expression systems for B. subtilis have been developed recently, and various target proteins have been recombinantly synthesized and purified using this host. In this chapter, we introduce a generic protocol to express a recombinant protein in B. subtilis. It includes protocols for (1) using our typical expression vector (plasmid pHT254) to introduce a target gene, (2) transformation of the target vector into B. subtilis, and (3) evaluation of the actual expression of a recombinant protein.

KEYWORDS:

Bacillus subtilis; Pgrac promoter; Pgrac100 promoter; pHT01 vector; pHT254

PMID:
28470615
DOI:
10.1007/978-1-4939-6887-9_21
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center