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Sci Rep. 2017 May 3;7(1):1390. doi: 10.1038/s41598-017-01500-1.

Interactive Toxicogenomics: Gene set discovery, clustering and analysis in Toxygates.

Author information

1
Level Five Co., Ltd., GYB Akihabara 3F, 2-25, Kanda-Sudacho, Chiyoda-ku, Tokyo, 101-0041, Japan. johan@level-five.jp.
2
Bioinformatics Project, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), 7-6-8, Asagi, Saito, Ibaraki-shi, Osaka, 567-0085, Japan. natsume@nibiohn.go.jp.
3
Toxicogenomics-informatics Project, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), 7-6-8, Asagi, Saito, Ibaraki-shi, Osaka, 567-0085, Japan.
4
Level Five Co., Ltd., GYB Akihabara 3F, 2-25, Kanda-Sudacho, Chiyoda-ku, Tokyo, 101-0041, Japan.
5
Bioinformatics Project, National Institutes of Biomedical Innovation, Health and Nutrition (NIBIOHN), 7-6-8, Asagi, Saito, Ibaraki-shi, Osaka, 567-0085, Japan. kenji@nibiohn.go.jp.

Abstract

Toxygates was originally released as a user-friendly interface to enhance the accessibility of the large-scale toxicogenomics database, Open TG-GATEs, generated by the Japanese Toxicogenomics Project. Since the original release, significant new functionality has been added to enable users to perform sophisticated computational analysis with only modest bioinformatics skills. The new features include an orthologous mode for data comparison among different species, interactive clustering and heatmap visualisation, enrichment analysis of gene sets, and user data uploading. In a case study, we use these new functions to study the hepatotoxicity of peroxisome proliferator-activated receptor alpha (PPARα) agonist WY-14643. Our findings suggest that WY-14643 caused hypertrophy in the bile duct by intracellular Ca2+ dysregulation, which resulted in the induction of genes in a non-canonical WNT/Ca2+ signalling pathway. With this new release of Toxygates, we provide a suite of tools that allow anyone to carry out in-depth analysis of toxicogenomics in Open TG-GATEs, and of any other dataset that is uploaded.

PMID:
28469246
PMCID:
PMC5431224
DOI:
10.1038/s41598-017-01500-1
[Indexed for MEDLINE]
Free PMC Article

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