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Electrophoresis. 2017 Sep;38(18):2313-2322. doi: 10.1002/elps.201700044. Epub 2017 May 22.

Synergic effects of sugar and caffeine on insulin-mediated metabolomic alterations after an acute consumption of soft drinks.

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Department of Chemistry, Faculty of Experimental Sciences, University of Huelva, Spain.
International Campus of Excellence CeiA3, University of Huelva, Spain.
Department of Pediatrics, Hospital Universitario Puerta del Mar, Cádiz, Spain.
Research Unit, Hospital Universitario Puerta del Mar, Cadiz, Spain.
Department of Biotechnology, Biomedicine and Public Health, Faculty of Medicine, University of Cádiz, Cádiz, Spain.
Department of Mother and Child Health and Radiology, Faculty of Medicine, University of Cádiz, Cádiz, Spain.
"Salus Infirmorum" Faculty of Nursing, University of Cádiz, Cádiz, Spain.
Instituto de Investigación Vitivinícola y Agroalimentario (IVAGRO), Puerto Real Campus, University of Cádiz, Puerto Real, Cádiz, Spain.


High sugar consumption elicits numerous deleterious effects on health by inducing insulin resistance, which is closely associated with the development of metabolic disorders such as obesity or type-2 diabetes. Furthermore, there is also growing evidence that caffeine may play an important role in the regulation of insulin release and the appearance of related metabolic impairments. Thus, the aim of this work was to investigate the impact of acute sugar and caffeine intake on the metabolic health status by using a metabolomic multi-platform based on the combination of flow injection mass spectrometry and ultra-high performance liquid chromatography mass spectrometry. To this end, we performed a randomized, crossover and double-blind intervention study with different soft drinks from the same brand. Numerous metabolomic changes were detected in serum samples over time after the intake of sugar-sweetened beverages, including energy-related metabolites, amino acids and lipids, thus demonstrating the intense effects provoked by acute sugar consumption on the organism during 3 h of follow-up. However, the most significant findings were observed after the co-ingestion of caffeine, which could be indicative of a synergic effect of this psychostimulant on insulin-mediated perturbations.


Caffeine; Hyperinsulinemia; Mass spectrometry; Metabolomics; Sugar

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