Format

Send to

Choose Destination
J Biol Chem. 2017 Jun 23;292(25):10696-10708. doi: 10.1074/jbc.M117.778480. Epub 2017 May 2.

Genetic metabolic complementation establishes a requirement for GDP-fucose in Leishmania.

Author information

1
From the Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110.
2
the Department of Biochemistry, University of Kentucky Medical Center, Lexington, Kentucky 40536, and.
3
the Division of Biological Chemistry and Drug Discovery, School of Life Science, University of Dundee, Dundee DD1 5EH, Scotland, United Kingdom.
4
From the Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110, stephen.beverley@wustl.edu.

Abstract

To survive in its sand fly vector, the trypanosomatid protozoan parasite Leishmania first attaches to the midgut to avoid excretion, but eventually it must detach for transmission by the next bite. In Leishmania major strain Friedlin, this is controlled by modifications of the stage-specific adhesin lipophosphoglycan (LPG). During differentiation to infective metacyclics, d-arabinopyranose (d-Arap) caps the LPG side-chain galactose residues, blocking interaction with the midgut lectin PpGalec, thereby leading to parasite detachment and transmission. Previously, we characterized two closely related L. major genes (FKP40 and AFKP80) encoding bifunctional proteins with kinase/pyrophosphorylase activities required for salvage and conversion of l-fucose and/or d-Arap into the nucleotide-sugar substrates required by glycosyltransferases. Whereas only AFKP80 yielded GDP-d-Arap from exogenous d-Arap, both proteins were able to salvage l-fucose to GDP-fucose. We now show that Δafkp80- null mutants ablated d-Arap modifications of LPG as predicted, whereas Δfkp40- null mutants resembled wild type (WT). Fucoconjugates had not been reported previously in L. major, but unexpectedly, we were unable to generate fkp40-/afkp80- double mutants, unless one of the A/FKPs was expressed ectopically. To test whether GDP-fucose itself was essential for Leishmania viability, we employed "genetic metabolite complementation." First, the trypanosome de novo pathway enzymes GDP-mannose dehydratase (GMD) and GDP-fucose synthetase (GMER) were expressed ectopically; from these cells, the Δfkp40-afkp80- double mutant was now readily obtained. As expected, the Δfkp40-afkp80-/+TbGMD-GMER line lacked the capacity to generate GDP-Arap, while synthesizing abundant GDP-fucose. These results establish a requirement for GDP-fucose for L. major viability and predict the existence of an essential fucoconjugate(s).

KEYWORDS:

D-Arabinopyranose; Fucose; Leishmania; Trypanosomatidae; adhesin; glycoconjugate; nucleotide sugar; parasite metabolism; protozoan; virulence factor

PMID:
28465349
PMCID:
PMC5481574
DOI:
10.1074/jbc.M117.778480
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center