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Adv Healthc Mater. 2017 Jun;6(12). doi: 10.1002/adhm.201601451. Epub 2017 May 2.

Extrusion Bioprinting of Shear-Thinning Gelatin Methacryloyl Bioinks.

Liu W1,2,3, Heinrich MA1,2,4, Zhou Y1,2, Akpek A1,2,5, Hu N1,2, Liu X1,2,6, Guan X1,2, Zhong Z1,2, Jin X3, Khademhosseini A1,2,7,8, Zhang YS1,2,7.

Author information

1
Biomaterials Innovation Research Center, Division of Engineering in Medicine, Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, 02139, USA.
2
Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA, 02139, USA.
3
Key Laboratory of Textile Science and Technology of the Ministry of Education, College of Textiles, Donghua University, Shanghai, 201620, China.
4
MIRA Institute of Biomedical Technology and Technical Medicine, Department of Developmental BioEngineering, University of Twente, Enschede, 7500AE, The Netherlands.
5
Department of Biomedical Engineering, Istanbul Yeni Yuzyil University, Istanbul, 34010, Turkey.
6
Key Laboratory for Biomechanics and Mechanobiology of the Ministry of Education, School of Biological Science and Medical Engineering, Beihang University, Beijing, 100191, China.
7
Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA.
8
Department of Bioindustrial Technologies, College of Animal Bioscience and Technology, Konkuk University, Seoul, 143-701, Republic of Korea.

Abstract

Bioprinting is an emerging technique for the fabrication of 3D cell-laden constructs. However, the progress for generating a 3D complex physiological microenvironment has been hampered by a lack of advanced cell-responsive bioinks that enable bioprinting with high structural fidelity, particularly in the case of extrusion-based bioprinting. Herein, this paper reports a novel strategy to directly bioprint cell-laden gelatin methacryloyl (GelMA) constructs using bioinks of GelMA physical gels (GPGs) achieved through a simple cooling process. Attributed to their shear-thinning and self-healing properties, the GPG bioinks can retain the shape and form integral structures after deposition, allowing for subsequent UV crosslinking for permanent stabilization. This paper shows the structural fidelity by bioprinting various 3D structures that are typically challenging to fabricate using conventional bioinks under extrusion modes. Moreover, the use of the GPG bioinks enables direct bioprinting of highly porous and soft constructs at relatively low concentrations (down to 3%) of GelMA. It is also demonstrated that the bioprinted constructs not only permit cell survival but also enhance cell proliferation as well as spreading at lower concentrations of the GPG bioinks. It is believed that such a strategy of bioprinting will provide many opportunities in convenient fabrication of 3D cell-laden constructs for applications in tissue engineering, regenerative medicine, and pharmaceutical screening.

KEYWORDS:

bioprinting; cell-laden; gelatin methacryloyl; hydrogels; tissue engineering

PMID:
28464555
PMCID:
PMC5545786
DOI:
10.1002/adhm.201601451
[Indexed for MEDLINE]
Free PMC Article

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