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Curr Biol. 2017 May 8;27(9):1387-1391. doi: 10.1016/j.cub.2017.03.069. Epub 2017 Apr 27.

Rapid Rates of Pol II Elongation in the Drosophila Embryo.

Author information

1
Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA. Electronic address: tfukaya@princeton.edu.
2
Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA.
3
Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, NJ 08544, USA; Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA. Electronic address: msl2@princeton.edu.

Abstract

Elongation of RNA polymerase II (Pol II) is thought to be an important mechanism for regulating gene expression [1]. We measured the first wave of de novo transcription in living Drosophila embryos using dual-fluorescence detection of nascent transcripts containing 5' MS2 and 3' PP7 RNA stem loops. Pol II elongation rates of 2.4-3.0 kb/min were observed, approximately twice as fast as earlier estimates [2-6]. The revised rates permit substantial levels of zygotic gene activity prior to the mid-blastula transition. We also provide evidence that variable rates of elongation are not a significant source of differential gene activity, suggesting that transcription initiation and Pol II release are the key determinants of gene control in development.

KEYWORDS:

Drosophila embryo; Pol II elongation; live imaging; transcription

PMID:
28457866
PMCID:
PMC5665007
DOI:
10.1016/j.cub.2017.03.069
[Indexed for MEDLINE]
Free PMC Article

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