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Carbohydr Polym. 2017 Jul 15;168:94-102. doi: 10.1016/j.carbpol.2017.03.062. Epub 2017 Mar 20.

Insights into cell wall structure of Sida hermaphrodita and its influence on recalcitrance.

Author information

1
Institute for Botany and Molecular Genetics, RWTH Aachen University, Worringer Weg 3, 52074 Aachen, Germany; Bioeconomy Science Center (BioSC), c/o Forschungszentrum Jülich, 52425 Jülich, Germany. Electronic address: damm@bio1.rwth-aachen.de.
2
Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Rd. Athens, GA, USA. Electronic address: spattathil@lallemand.com.
3
Institute of Bio- and Geosciences, IBG-2: Plant Sciences, Forschungszentrum Jülich, Leo- Brandt-Straße, 52425 Jülich, Germany. Electronic address: m.guenl@fz-juelich.de.
4
Bioeconomy Science Center (BioSC), c/o Forschungszentrum Jülich, 52425 Jülich, Germany; Institute of Bio- and Geosciences, IBG-2: Plant Sciences, Forschungszentrum Jülich, Leo- Brandt-Straße, 52425 Jülich, Germany. Electronic address: n.d.jablonowski@fz-juelich.de.
5
Complex Carbohydrate Research Center, The University of Georgia, 315 Riverbend Rd. Athens, GA, USA. Electronic address: mao@ccrc.uga.edu.
6
Institute for Botany and Molecular Genetics, RWTH Aachen University, Worringer Weg 3, 52074 Aachen, Germany. Electronic address: kathrin.gruen@rwth-aachen.de.
7
Institute for Technical and Macromolecular Chemistry, RWTH Aachen University, Worringer Weg 1-2, 52074 Aachen Germany. Electronic address: grande@itmc.rwth-aachen.de.
8
Institute for Technical and Macromolecular Chemistry, RWTH Aachen University, Worringer Weg 1-2, 52074 Aachen Germany; Max-Planck-Institut für Kohlenforschung, 45470 Mülheim an der Ruhr, Germany. Electronic address: leitner@itmc.rwth-aachen.de.
9
Bioeconomy Science Center (BioSC), c/o Forschungszentrum Jülich, 52425 Jülich, Germany; Institute of Bio- and Geosciences, IBG-2: Plant Sciences, Forschungszentrum Jülich, Leo- Brandt-Straße, 52425 Jülich, Germany. Electronic address: u.schurr@fz-juelich.de.
10
Institute for Botany and Molecular Genetics, RWTH Aachen University, Worringer Weg 3, 52074 Aachen, Germany; Bioeconomy Science Center (BioSC), c/o Forschungszentrum Jülich, 52425 Jülich, Germany; Institute of Bio- and Geosciences, IBG-2: Plant Sciences, Forschungszentrum Jülich, Leo- Brandt-Straße, 52425 Jülich, Germany. Electronic address: usadel@bio1.rwth-aachen.de.
11
Institute for Botany and Molecular Genetics, RWTH Aachen University, Worringer Weg 3, 52074 Aachen, Germany; Bioeconomy Science Center (BioSC), c/o Forschungszentrum Jülich, 52425 Jülich, Germany. Electronic address: h.klose@bio1.rwth-aachen.de.

Abstract

The perennial plant Sida hermaphrodita (Sida) is attracting attention as potential energy crop. Here, the first detailed view on non-cellulosic Sida cell wall polysaccharide composition, structure and architecture is given. Cell walls were prepared from Sida stems and sequentially extracted with aqueous buffers and alkali. The structures of the quantitatively predominant polysaccharides present in each fraction were determined by biochemical characterization, glycome profiling and mass spectrometry. The amounts of glucose released by Accellerase-1500® treatment of the cell wall and the cell wall residue remaining after each extraction were used to assess the roles of pectin and hemicellulose in the recalcitrance of Sida biomass. 4-O-Methyl glucuronoxylan with a low proportion of side substitutions was identified as the major non-cellulosic glycan component of Sida stem cell walls. Pectic polysaccharides and xylans were found to be associated with lignin, suggesting that these polysaccharides have roles in Sida cell wall recalcitrance to enzymatic hydrolysis.

KEYWORDS:

Glucuronoxylan; Lignocellulose utilization; Non-cellulosic polysaccharides; Pectin; Plant cell wall; Sida hermaphrodita

PMID:
28457468
DOI:
10.1016/j.carbpol.2017.03.062
[Indexed for MEDLINE]

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