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Methods Mol Biol. 2017;1594:191-203. doi: 10.1007/978-1-4939-6934-0_12.

Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry.

Author information

1
Clinical Chemistry, Department of Clinical and Experimental Medicine, Linköping University, SE-58185, Linköping, Sweden. anna.sodergren@liu.se.
2
Department of Clinical Chemistry and Clinical and Experimental Medicine, Linköping University, Linköping, Sweden.
3
Department of Clinical Medicine, Örebro University, Örebro, Sweden.

Abstract

Flow cytometry is a method that allows high throughput analysis of individual cells in suspension. By inclusion of fluorescently labelled antibodies, it is possible to analyze the abundance of one or more surface antigens, such as LAMP-1, without prior lysis of cells. Here we describe the special considerations required for the investigation of lysosomal exocytosis from platelets analyzed with flow cytometry.

KEYWORDS:

Flow cytometry; Granule; Lysosomal exocytosis; Lysosomal-associated membrane protein-1 (LAMP-1); Lysosome; Platelet; Platelet activation; Platelet secretion

PMID:
28456984
DOI:
10.1007/978-1-4939-6934-0_12
[Indexed for MEDLINE]

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