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Biochem Biophys Res Commun. 2017 Jun 10;487(4):847-855. doi: 10.1016/j.bbrc.2017.04.140. Epub 2017 Apr 26.

Constitutive ω-3 fatty acid production in fat-1 transgenic mice and docosahexaenoic acid administration to wild type mice protect against 2,4,6-trinitrobenzene sulfonic acid-induced colitis.

Author information

1
Tumor Microenvironment Global Core Research Center, College of Pharmacy, Seoul National University, Seoul 08826, Republic of Korea.
2
Laboratory for Lipid Medicine and Technology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02129, USA.
3
CHA Cancer Prevention Research Center, CHA Bio Complex, Seongnam, Gyunggi-do, 13488, Republic of Korea; Digestive Disease Center, CHA University Bundang Medical Center, Seongnam, Gyunggi-do, 13496, Republic of Korea.
4
Tumor Microenvironment Global Core Research Center, College of Pharmacy, Seoul National University, Seoul 08826, Republic of Korea. Electronic address: surh@snu.ac.kr.

Abstract

Omega-3 (ω-3) polyunsaturated fatty acids (PUFAs) are known to have strong anti-inflammatory effects. In the present study, we investigated the protective effects of ω-3 PUFAs on experimentally induced murine colitis. Intrarectal administration of 2.5% 2,4,6-trinitrobenzene sulfonic acid (TNBS) caused inflammation in the colon of wild type mice, but this was less severe in fat-1 transgenic mice that constitutively produce ω-3 PUFAs from ω-6 PUFAs. The intraperitoneal administration of docosahexaenoic acid (DHA), a representative ω-3 PUFA, was also protective against TNBS-induced murine colitis. In addition, endogenously formed and exogenously introduced ω-3 PUFAs attenuated the production of malondialdehyde and 4-hydroxynonenal in the colon of TNBS-treated mice. The effective protection against inflammatory and oxidative colonic tissue damages in fat-1 and DHA-treated mice was associated with suppression of NF-κB activation and cyclooxygenase-2 expression and with elevated activation of Nrf2 and upregulation of its target gene, heme oxygenase-1. Taken together, these results provide mechanistic basis of protective action of ω-3 fatty PUFAs against experimental colitis.

KEYWORDS:

2,4,6-Trinitrobenzene sulfonic acid; Colitis; Docosahexaenoic acid; fat-1 Transgenic mice; ω-3 Polyunsaturated fatty acids

PMID:
28456627
DOI:
10.1016/j.bbrc.2017.04.140
[Indexed for MEDLINE]

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