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Trends Biotechnol. 2017 Aug;35(8):728-742. doi: 10.1016/j.tibtech.2017.03.013. Epub 2017 Apr 26.

Multiplexed Point-of-Care Testing - xPOCT.

Author information

1
University of Freiburg, Department of Microsystems Engineering (IMTEK), Laboratory for Sensors, Georges-Koehler-Allee 103, 79110 Freiburg, Germany; University of Freiburg, Freiburg Materials Research Center (FMF), Stefan-Meier-Straße 21, 79104 Freiburg, Germany. Electronic address: dincer@imtek.de.
2
University of Freiburg, Department of Microsystems Engineering (IMTEK), Laboratory for Sensors, Georges-Koehler-Allee 103, 79110 Freiburg, Germany.
3
ETH Zurich, Department of Biosystems Science and Engineering, Bioanalytics Group, Mattenstrasse 26, 4058 Basel, Switzerland.
4
University of Freiburg, Department of Microsystems Engineering (IMTEK), Laboratory for Sensors, Georges-Koehler-Allee 103, 79110 Freiburg, Germany; University of Freiburg, Freiburg Materials Research Center (FMF), Stefan-Meier-Straße 21, 79104 Freiburg, Germany.

Abstract

Multiplexed point-of-care testing (xPOCT), which is simultaneous on-site detection of different analytes from a single specimen, has recently gained increasing importance for clinical diagnostics, with emerging applications in resource-limited settings (such as in the developing world, in doctors' offices, or directly at home). Nevertheless, only single-analyte approaches are typically considered as the major paradigm in many reviews of point-of-care testing. Here, we comprehensively review the present diagnostic systems and techniques for xPOCT applications. Different multiplexing technologies (e.g., bead- or array-based systems) are considered along with their detection methods (e.g., electrochemical or optical). We also address the unmet needs and challenges of xPOCT. Finally, we critically summarize the in-field applicability and the future perspectives of the presented approaches.

KEYWORDS:

lab-on-a-chip (LOC); microfluidics; multianalyte analysis; multiplexing; on-site testing; point-of-care testing (POCT)

PMID:
28456344
PMCID:
PMC5538621
DOI:
10.1016/j.tibtech.2017.03.013
[Indexed for MEDLINE]
Free PMC Article

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