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Biomed Pharmacother. 2017 Jul;91:138-146. doi: 10.1016/j.biopha.2017.04.067. Epub 2017 Apr 27.

The enhancement mechanism of wine-processed Radix Scutellaria on NTG-induced migraine rats.

Author information

1
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, JS 210009, China.
2
School of Pharmacy, Queen's University of Belfast, BT7 1NN, UK.
3
The people's Hospital of Zhangqiu, Zhangqiu 250200, China.
4
Tang Center of Herbal Medicine Research and Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL 60637, USA.
5
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, JS 210009, China. Electronic address: guochangrun@126.com.
6
State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, JS 210009, China; Tang Center of Herbal Medicine Research and Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL 60637, USA. Electronic address: zhangchunfeng67@163.com.

Abstract

To elucidate the increasing dissolution and enhancement mechanism of wine-processed Radix Scutellaria (RS) by fractal theory in nitroglycerin (NTG)-induced migraine rats. We prepared three RS from the process with 10% (S1), 15% (S2), 20% (S3) (v/m) rice wine. Mercury intrusion porosimetry and scanning electron microscope were employed to explore the internal structure of RS and the components dissolution of RS was analyzed by HPLC. Rats were randomly allocated into following groups and orally given different solutions for 10days: normal group (NOR, normal saline), model group (MOD, normal saline), Tianshu capsule group (TSC, 0.425mg/kg), ibuprofen group (IBU, 0.0821mg/kg), crude RS group (CRU, 1.04mg/kg) and wine-processed RS group (WP, 1.04mg/kg) followed by bolus subcutaneously injection of NTG (10mg/kg) to induce migraine model except NOR. Biochemical indexes (nitric oxide-NO, calcitonin-gene-related peptide-CGRP, and endothelin-ET) and c-fos positive cells were measured with commercial kits and immunohistochemical method, separately. Total surface area significantly increased in wine-processed RS (p<0.05) while fractal dimension markedly decreased (p<0.05) compared with crude RS. Additionally, S3 owned the highest increase of dissolution including the percentage increase of total extract, total flavonoids and main compounds (all p<0.05 vs S1 and S2). Pharmacodynamic data showed c-fos positive cells significantly decreased (p<0.05) in WP compared with MOD and the level of NO, CGRP, ET in WP was better than that of CRU. Wine-processed RS could be a promising candidate medicine for migraine treatment due to its increased component dissolution.

KEYWORDS:

Biochemical indexes; Component dissolution; Fractal theory; Wine-processed Radix Scutellaria

PMID:
28456113
DOI:
10.1016/j.biopha.2017.04.067
[Indexed for MEDLINE]

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