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Plant Physiol. 2017 Jun;174(2):1151-1166. doi: 10.1104/pp.16.01625. Epub 2017 Apr 28.

A Rice PECTATE LYASE-LIKE Gene Is Required for Plant Growth and Leaf Senescence.

Leng Y1,2,3,4,5, Yang Y1,2,3,4,5, Ren D1,2,3,4,5, Huang L1,2,3,4,5, Dai L1,2,3,4,5, Wang Y1,2,3,4,5, Chen L1,2,3,4,5, Tu Z1,2,3,4,5, Gao Y1,2,3,4,5, Li X1,2,3,4,5, Zhu L1,2,3,4,5, Hu J1,2,3,4,5, Zhang G1,2,3,4,5, Gao Z1,2,3,4,5, Guo L1,2,3,4,5, Kong Z1,2,3,4,5, Lin Y1,2,3,4,5, Qian Q6,7,8,9,10, Zeng D6,7,8,9,10.

Author information

1
State Key Lab for Rice Biology, China National Rice Research Institute, Hangzhou 310006, China (Yu.L., Y.Y., D.R., L.H., L.D., Y.W., L.C., Z.T., Y.G., L.Z., J.H., G.Z., Z.G., L.G., Q.Q., D.Z.).
2
National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China (Yu.L., L.D., L.C., Yo.L.).
3
National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China (X.L.), and.
4
Institute of Microbiology, Chinese Academy of Sciences, State Key Laboratory of Plant Genomics, Beijing 100101, China (Z.K.).
5
State Key Lab for Rice Biology, China National Rice Research Institute, Hangzhou 310006, China (Yu.L., Y.Y., D.R., L.H., L.D., Y.W., L.C., Z.T., Y.G., L.Z., J.H., G.Z., Z.G., L.G., Q.Q., D.Z.), National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China (Yu.L., L.D., L.C., Yo.L.), National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China (X.L.), and Institute of Microbiology, Chinese Academy of Sciences, State Key Laboratory of Plant Genomics, Beijing 100101, China (Z.K.).
6
State Key Lab for Rice Biology, China National Rice Research Institute, Hangzhou 310006, China (Yu.L., Y.Y., D.R., L.H., L.D., Y.W., L.C., Z.T., Y.G., L.Z., J.H., G.Z., Z.G., L.G., Q.Q., D.Z.), dalizeng@126.com qianqian188@hotmail.com.
7
National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China (Yu.L., L.D., L.C., Yo.L.), dalizeng@126.com qianqian188@hotmail.com.
8
National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China (X.L.), and dalizeng@126.com qianqian188@hotmail.com.
9
Institute of Microbiology, Chinese Academy of Sciences, State Key Laboratory of Plant Genomics, Beijing 100101, China (Z.K.) dalizeng@126.com qianqian188@hotmail.com.
10
State Key Lab for Rice Biology, China National Rice Research Institute, Hangzhou 310006, China (Yu.L., Y.Y., D.R., L.H., L.D., Y.W., L.C., Z.T., Y.G., L.Z., J.H., G.Z., Z.G., L.G., Q.Q., D.Z.), National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China (Yu.L., L.D., L.C., Yo.L.), National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China (X.L.), and Institute of Microbiology, Chinese Academy of Sciences, State Key Laboratory of Plant Genomics, Beijing 100101, China (Z.K.) dalizeng@126.com qianqian188@hotmail.com.

Abstract

To better understand the molecular mechanisms behind plant growth and leaf senescence in monocot plants, we identified a mutant exhibiting dwarfism and an early-senescence leaf phenotype, termed dwarf and early-senescence leaf1 (del1). Histological analysis showed that the abnormal growth was caused by a reduction in cell number. Further investigation revealed that the decline in cell number in del1 was affected by the cell cycle. Physiological analysis, transmission electron microscopy, and TUNEL assays showed that leaf senescence was triggered by the accumulation of reactive oxygen species. The DEL1 gene was cloned using a map-based approach. It was shown to encode a pectate lyase (PEL) precursor that contains a PelC domain. DEL1 contains all the conserved residues of PEL and has strong similarity with plant PelC. DEL1 is expressed in all tissues but predominantly in elongating tissues. Functional analysis revealed that mutation of DEL1 decreased the total PEL enzymatic activity, increased the degree of methylesterified homogalacturonan, and altered the cell wall composition and structure. In addition, transcriptome assay revealed that a set of cell wall function- and senescence-related gene expression was altered in del1 plants. Our research indicates that DEL1 is involved in both the maintenance of normal cell division and the induction of leaf senescence. These findings reveal a new molecular mechanism for plant growth and leaf senescence mediated by PECTATE LYASE-LIKE genes.

PMID:
28455404
PMCID:
PMC5462006
DOI:
10.1104/pp.16.01625
[Indexed for MEDLINE]
Free PMC Article

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