Send to

Choose Destination
Oncol Lett. 2017 Mar;13(3):1958-1964. doi: 10.3892/ol.2017.5608. Epub 2017 Jan 17.

miR-455-5p functions as a potential oncogene by targeting galectin-9 in colon cancer.

Author information

Department of Clinical Laboratory, Union Hospital, Tongji Medical Collage, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China.
Department of Blood Transfusion, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200092, P.R. China.


Although there is evidence that galectin-9 is a critical factor in health and disease, the upstream regulatory microRNA (miRNA or miR) of the protein remains poorly defined. miR-455-5p is characterized as a tumor-associated miRNA in cancer research. However, the actual role of miR-455-5p with respect to inhibiting or promoting tumorigenesis in colon cancer is unclear. The present study aimed to investigate the expression, role and target regulation association of galectin-9 and miR-455-5p in colon cancer. Western blot analysis and reverse transcription-quantitative polymerase chain reaction were used for the detection of the expression levels of galectin-9 and miRNAs. Cell Counting kit-8 test was used for the evaluation of cell proliferation, while flow cytometry was used for cell apoptosis analysis. A potential interaction between galectin-9 and miR-455-5p was predicted by target prediction programs and confirmed by luciferase assay and transfection with miRNA mimics. The present study revealed that elevated expression of galectin-9 and miR-455-5p in colon cancer was associated with HT29 cell proliferation and apoptosis. Furthermore, the present study demonstrated that miR-455-5p reduced galectin-9 expression by directly targeting its 3'-untranslated region. These data suggest that miR-455-5p functions as a potential oncogene in colon cancer by targeting galectin-9.


colon cancer; galectin-9; miR-455-5p; microRNA

Supplemental Content

Loading ...
Support Center