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Adipocyte. 2017 Jan 2;6(1):33-39. doi: 10.1080/21623945.2016.1263777. Epub 2016 Dec 7.

Fasting rapidly increases fatty acid oxidation in white adipose tissue of young broiler chickens.

Author information

1
a Department of Animal Science , University of Tennessee , Knoxville , TN , USA.
2
b Department of Human Nutrition , Foods and Exercise, Virginia Tech , Blacksburg , VA , USA.
3
c The Metabolic Phenotyping Core at Virginia Tech, Virginia Tech , Blacksburg , VA , USA.

Abstract

Upregulating the fatty acid oxidation capacity of white adipose tissue in mice protects against diet-induced obesity, inflammation and insulin resistance. Part of this capacity results from induction of brown-like adipocytes within classical white depots, making it difficult to determine the oxidative contribution of the more abundant white adipocytes. Avian genomes lack a gene for uncoupling protein 1 and are devoid of brown adipose cells, making them a useful model in which to study white adipocyte metabolism in vivo. We recently reported that a brief (5 hour) period of fasting significantly upregulated many genes involved in mitochondrial and peroxisomal fatty acid oxidation pathways in white adipose tissue of young broiler chickens. The objective of this study was to determine if the effects on gene expression manifested in increased rates of fatty acid oxidation. Abdominal adipose tissue was collected from 21 day-old broiler chicks that were fasted for 3, 5 or 7 hours or fed ad libitum (controls). Fatty acid oxidation was determined by measuring and summing 14CO2 production and 14C-labeled acid-soluble metabolites from the oxidation of [1-14C] palmitic acid. Fasting induced a progressive increase in complete fatty acid oxidation and citrate synthase activity relative to controls. These results confirm that fatty acid oxidation in white adipose tissue is dynamically controlled by nutritional status. Identifying the underlying mechanism may provide new therapeutic targets through which to increase fatty acid oxidation in situ and protect against the detrimental effects of excess free fatty acids on adipocyte insulin sensitivity.

KEYWORDS:

adipose; fasting; fatty acid oxidation

PMID:
28452587
PMCID:
PMC5358707
DOI:
10.1080/21623945.2016.1263777
[Indexed for MEDLINE]
Free PMC Article

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