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Arch Virol. 2017 Aug;162(8):2409-2413. doi: 10.1007/s00705-017-3378-1. Epub 2017 Apr 27.

A single L288I substitution in the fusion protein of bovine parainfluenza virus type 3 enhances virus growth in semi-suitable cell lines.

Author information

1
Laboratory of Environmental Microbiology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, 305-8575, Japan.
2
Biologics Production, Center for Animal Disease Control and Prevention, National Institute of Animal Health, NARO, Tsukuba, Ibaraki, 305-0856, Japan.
3
Viral Diseases and Epidemiology Research Division, National Institute of Animal Health, NARO, Tsukuba, Ibaraki, 305-0856, Japan.
4
Laboratory of Environmental Microbiology, Faculty of Medicine, University of Tsukuba, Tsukuba, Ibaraki, 305-8575, Japan. ktakeuch@md.tsukuba.ac.jp.

Abstract

The bovine parainfluenza virus type 3 BN-CE vaccine strain was obtained by serial passage of the BN-1 strain in chicken embryonic fibroblasts (CEF). We previously identified a substitution (L288I) in the fusion (F) protein between the two strains. To examine the effect of the substitution on CEF adaptation and attenuation, we generated a recombinant BN-1 strain with the L288I substitution in the F protein (FL288I-EGFP). FL288I-EGFP replicated more efficiently than a recombinant BN-1 strain (wt-EGFP) in semi-suitable cell lines, suggesting that the L288I substitution was established in the BN-1 strain during the process of adaptation in CEF.

KEYWORDS:

Bovine parainfluenza virus type 3; Cell-specific adaptation; Fusion protein

PMID:
28451903
DOI:
10.1007/s00705-017-3378-1
[Indexed for MEDLINE]

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