Format

Send to

Choose Destination
Plant Cell Rep. 2017 Jul;36(7):1137-1157. doi: 10.1007/s00299-017-2144-x. Epub 2017 Apr 27.

Analysis of the potato calcium-dependent protein kinase family and characterization of StCDPK7, a member induced upon infection with Phytophthora infestans.

Author information

1
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor Torres" (INGEBI-CONICET) Vuelta de Obligado 2490, 2do piso, Ciudad Autónoma de Buenos Aires, Argentina.
2
Departamento de Fisiología, Biología Molecular y Celular Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina.
3
Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor Torres" (INGEBI-CONICET) Vuelta de Obligado 2490, 2do piso, Ciudad Autónoma de Buenos Aires, Argentina. rulloa@dna.uba.ar.
4
Departamento de Química Biológica, Universidad de Buenos Aires, Ciudad Autónoma de Buenos Aires, Argentina. rulloa@dna.uba.ar.

Abstract

We describe the potato CDPK family and place StCDPK7 as a player in potato response to Phytophthora infestans infection, identifying phenylalanine ammonia lyase as its specific phosphorylation target in vitro. Calcium-dependent protein kinases (CDPKs) decode calcium (Ca2+) signals and activate different signaling pathways involved in hormone signaling, plant growth, development, and both abiotic and biotic stress responses. In this study, we describe the potato CDPK/CRK multigene family; bioinformatic analysis allowed us to identify 20 new CDPK isoforms, three CDPK-related kinases (CRKs), and a CDPK-like kinase. Phylogenetic analysis indicated that 26 StCDPKs can be classified into four groups, whose members are predicted to undergo different acylation patterns and exhibited diverse expression levels in different tissues and in response to various stimuli. With the aim of characterizing those members that are particularly involved in plant-pathogen interaction, we focused on StCDPK7. Tissue expression profile revealed that StCDPK7 transcript levels are high in swollen stolons, roots, and mini tubers. Moreover, its expression is induced upon Phytophthora infestans infection in systemic leaves. Transient expression assays showed that StCDPK7 displays a cytosolic/nuclear localization in spite of having a predicted chloroplast transit peptide. The recombinant protein, StCDPK7:6xHis, is an active Ca2+-dependent protein kinase that can phosphorylate phenylalanine ammonia lyase, an enzyme involved in plant defense response. The analysis of the potato CDPK family provides the first step towards the identification of CDPK isoforms involved in biotic stress. StCDPK7 emerges as a relevant player that could be manipulated to deploy disease resistance in potato crops.

KEYWORDS:

Calcium-dependent protein kinases; Phenylalanine ammonia lyase; Phytophthora infestans; Solanum tuberosum

PMID:
28451820
DOI:
10.1007/s00299-017-2144-x
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center