Format

Send to

Choose Destination
Chem Sci. 2017 Feb 1;8(2):1658-1664. doi: 10.1039/c6sc04086a. Epub 2016 Nov 16.

Design of a synthetic luminescent probe from a biomolecule binding domain: selective detection of AU-rich mRNA sequences.

Author information

1
Univ. Grenoble Alpes , LCBM/PMB , F-38000 Grenoble , France.
2
CNRS , LCBM/PMB , UMR 5249 , F-38000 Grenoble , France.
3
CEA , BIG-CBM , PMB , F-38000 Grenoble , France . Email: olivier.seneque@cea.fr.
4
Department of Pharmaceutical Sciences , School of Pharmacy , University of Maryland , Baltimore , Maryland 21201-1180 , USA . Email: smichel@rx.umaryland.edu.
5
Univ. Grenoble Alpes , INAC-SyMMES , F-38000 Grenoble , France.
6
CEA , INAC-SyMMES , F-38000 Grenoble , France.

Abstract

We report the design of a luminescent sensor based upon the zinc finger (ZF) protein TIS11d, that allows for the selective time-resolved detection of the UUAUUUAUU sequence of the 3'-untranslated region of messenger RNA. This sensor is composed of the tandem ZF RNA binding domain of TIS11d functionalized with a luminescent Tb3+ complex on one of the ZFs and a sensitizing antenna on the other. This work provides the proof of principle that an RNA binding protein can be re-engineered as an RNA sensor and, more generally, that tunable synthetic luminescent probes for biomolecules can be obtained by modifying biomolecule-binding domains.

Supplemental Content

Full text links

Icon for Royal Society of Chemistry Icon for PubMed Central
Loading ...
Support Center