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J Chromatogr. 1988 Jun 29;443:3-11.

High-performance immobilized-metal-ion affinity chromatography of peptides and proteins.

Author information

1
Institute of Biochemistry, University of Uppsala, Sweden.

Abstract

The present status of the undeveloped branch of high-performance immobilized-metal-ion affinity chromatography (HPIMAC) is reviewed. As demonstrated, high resolution of peptides and protein mixtures can be obtained. Under specified conditions, more than 1000 runs can be made repeatedly on a single column without much change in pattern or recovery. Occasionally, 10(3)- to 10(4)-fold purification of a protein can be obtained in a single chromatographic run.

PMID:
2844839
DOI:
10.1016/s0021-9673(00)94778-2
[Indexed for MEDLINE]

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