Format

Send to

Choose Destination
Pharm Biol. 2017 Dec;55(1):1671-1678. doi: 10.1080/13880209.2017.1318293.

Centella asiatica enhances hepatic antioxidant status and regulates hepatic inflammatory cytokines in type 2 diabetic rats.

Author information

1
a Discipline of Biochemistry , School of Life Sciences, University of KwaZulu-Natal , Durban , South Africa.
2
b DST/NRF Centre of Excellence for Biomedical Tuberculosis Research and MRC Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences , Stellenbosch University , Tygerberg , South Africa.
3
c Department of Biomedical Sciences, Phytomedicine and Diabetes Research Group , Oxidative Stress Research Centre, Cape Peninsula University of Technology , Bellville , South Africa.

Abstract

CONTEXT:

Neutralizing the over-activation of oxidative stress and inflammation remains an important goal in the management of type 2 diabetes mellitus (T2DM). Centella asiatica (L.) Urban (Apiaceae) (CA) has been used in traditional folklore in Africa and Asia to treat various ailments including diabetes.

OBJECTIVE:

We investigated the hepatic antioxidant and anti-inflammatory potential of methanol extract of CA leaves in T2DM.

MATERIALS AND METHODS:

T2DM was induced in male Sprague-Dawley rats with 10% fructose in drinking water for 14 days followed by a single intraperitoneal injection of streptozotocin (40 mg/kg b.wt). Hepatic oxidant/antioxidant status was assessed by measuring the concentrations of malondialdehyde (MDA), ferric reducing antioxidant power (FRAP), oxygen radical absorbance capacity (ORAC), Trolox equivalent antioxidant capacity (TEAC), reduced glutathione (GSH) and activities of glutathione S-transferase (GST) and glutathione peroxidase (GPX). The concentrations of cytokines IL-1β, IL-4, IL-6, IL-10, MCP-1 and TNF-α in the liver were determined.

RESULTS:

Diabetes increased MDA formed (47%) and reduced FRAP (20%), TEAC (15%), GSH levels (32%), significantly; decreased GST and GPX activities in the liver and elevated levels of cytokines studied. Treatment of diabetic rats with 500 mg/kg b.wt CA for 14 days decreased MDA (44%); elevated FRAP (15%) and GSH (131%) levels and increased the activities of GST and GPX by 16%. Hepatic concentrations of IL-1β, MCP-1 and TNF-α in DCA group were reduced to 68%, 75% and 63% of DC values, respectively.

CONCLUSIONS:

The antioxidant and anti-inflammatory properties of CA may protect tissues such as the liver from diabetes-induced oxidative damage.

KEYWORDS:

Apiaceae; Diabetes; lipids; liver; oxidative stress

PMID:
28447512
PMCID:
PMC6130484
DOI:
10.1080/13880209.2017.1318293
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Taylor & Francis Icon for PubMed Central
Loading ...
Support Center