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Stem Cell Res Ther. 2017 Apr 26;8(1):94. doi: 10.1186/s13287-017-0558-6.

The therapeutic potential of three-dimensional multipotent mesenchymal stromal cell spheroids.

Author information

1
Department of Biomaterials and Biophysical Methods, Institute of Experimental Medicine AS CR v. v. i, Vídeňská 1083, 14220, Prague 4-Krč, Czech Republic. yuri.petrenko@biomed.cas.cz.
2
Department of Neuroscience, Charles University, Second Faculty of Medicine, V Uvalu 84, 15006, Prague, Czech Republic.
3
Department of Biomaterials and Biophysical Methods, Institute of Experimental Medicine AS CR v. v. i, Vídeňská 1083, 14220, Prague 4-Krč, Czech Republic.

Abstract

The efficiency of clinical trials involving transplantation of multipotent mesenchymal stromal cells (MSCs) is often insufficient due to harsh conditions present within the target tissue including hypoxia, low nutrient supply as well as inflammatory reactions. This indicates the necessity for optimization of cell-based therapy approaches which might include either modification of the cell manufacturing process or specific cell pretreatment procedures prior to transplantation. Recent reports confirm evidence that the aggregation of MSCs into three-dimensional (3D) multicellular spheroids results in enhancement of the overall therapeutic potential of cells, by improving the anti-inflammatory and angiogenic properties, stemness and survival of MSCs after transplantation. Such an MSCs spheroid generation approach may open new opportunities for the enlargement of MSCs applications in clinical research and therapy. However, the unification and optimization of 3D spheroid generation techniques, including the selection of appropriate clinical-grade culture conditions and methods for their large-scale production, are still of great importance. The current review addresses questions regarding therapeutic-associated properties of 3D multicellular MSCs spheroids in vitro and during preclinical animal studies, with special attention to the possibilities of translating these research achievements toward further clinical manufacturing and applications.

KEYWORDS:

Clinical-grade manufacturing; Multipotent mesenchymal stromal cells; Paracrine activity; Pre-clinical studies; Survival; Therapeutic efficiency; Three-dimensional spheroids

PMID:
28446248
PMCID:
PMC5406927
DOI:
10.1186/s13287-017-0558-6
[Indexed for MEDLINE]
Free PMC Article

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