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J Biomed Mater Res A. 2017 Aug;105(8):2291-2298. doi: 10.1002/jbm.a.36095. Epub 2017 May 17.

Investigating the effect of fibulin-1 on the differentiation of human nasal inferior turbinate-derived mesenchymal stem cells into osteoblasts.

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Department of Biochemistry, Inha University School of Medicine, Incheon, 22212, Korea.
Department of Biomedical Science, The Catholic University of Korea, College of Medicine, Seoul Korea.
Department of Otolaryngology-Head and Neck Surgery, The Catholic University of Korea College of Medicine, Seoul Korea.
Department of Chemistry, University of Aveiro, Aveiro, Portugal.


Many extracellular matrix proteins have positive influences on the adhesion, proliferation, and differentiation of stem cells into specific cell linages. Fibulin-1 (FBLN1), a member of a growing family of extracellular glycoproteins, contributes to the structure of the extracellular matrix. Here, we investigated the effect of FBLN1 on the ability of human nasal inferior turbinate-derived mesenchymal stem cells (hTMSCs) to undergo osteogenic differentiation. After we generated recombinant FBLN1, the characteristics of FBLN1-treated hTMSCs were evaluated using MTT assay, ALP and mineralization activities, and quantitative real-time PCR. FBLN1 significantly enhanced the adhesion activity (p < 0.001) and proliferation of hTMSCs (p < 0.05). The ALP and mineralization activities of cells were dramatically increased (p < 0.01) after 9 and 12 days of FBLN1 treatment, respectively. This indicated the ability of FBLN1 to induce hTMSCs to differentiate into osteoblasts. Furthermore, increasing the mRNA levels of osteogenic marker genes, such as a transcriptional coactivator with a PDZ-binding motif (TAZ), alkaline phosphatase (ALP), collagen type I (Col I), and osteocalcin (OCN), improved bone repair and regeneration.


extracellular matrix proteins; fibulin-1; hTMSCs; osteoblast; osteogenic differentiation

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