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Biochimie. 1988 Apr;70(4):559-66.

Construction of a vector plasmid family and its use for molecular cloning in Streptococcus lactis.

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Institut National de la Recherche Agronomique, Laboratoire de Recherches de Technologie Laitière, Rennes, France.


Cloning vector plasmids have been constructed on the basis of the broad host range plasmid pAM beta 1 and used for the cloning of a nisin resistance determinant in Streptococcus lactis. They incorporate several desirable features for gene cloning in S. lactis and other transformable Gram-positive bacteria. They carry an easily selectable erythromycin resistance marker, are present at low (6-9) or high (45-85) copy number in S. lactis and possess a convenient polyrestriction site sequence. A significant advantage of these plasmids is their capability to carry and stably maintain very large cloned DNA fragments (up to 30 kilobases).

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