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Biochemistry. 1988 Jun 28;27(13):4706-11.

Chromatin domain surrounding the human interferon-beta gene as defined by scaffold-attached regions.

Author information

1
Gesellschaft für Biotechnologische Forschung mbH, Genetik von Eukaryonten, Braunschweig, West Germany.

Abstract

Regions attached to the nuclear scaffold have been traced after transfecting a 36-kilobase (kb) piece of DNA, surrounding the human interferon-beta gene, into mouse L-cells. An extended attached region starts 1.7 kb upstream from the gene and a moderate binding site immediately downstream. These findings could be confirmed by reconstitution experiments in vitro which predict another scaffold-attached region (SAR) starting 12 kb downstream from the gene. Since no other transcripts originate from DNA between the major SARs, these elements could be involved in interferon gene regulation.

PMID:
2844236
DOI:
10.1021/bi00413a019
[Indexed for MEDLINE]

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