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Dev Cell. 2017 Apr 24;41(2):195-203.e3. doi: 10.1016/j.devcel.2017.03.024.

PCP and SAX-3/Robo Pathways Cooperate to Regulate Convergent Extension-Based Nerve Cord Assembly in C. elegans.

Author information

1
Developmental Biology Program, Sloan Kettering Institute, New York, NY 10065, USA.
2
Neuroscience Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8M5, Canada; Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada.
3
Neuroscience Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8M5, Canada.
4
Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada.
5
Regenerative Medicine Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8L6, Canada.
6
Developmental Biology Program, Sloan Kettering Institute, New York, NY 10065, USA. Electronic address: baoz@mskcc.org.
7
Neuroscience Program, Ottawa Hospital Research Institute, Ottawa, ON K1H 8M5, Canada; Department of Cellular and Molecular Medicine, University of Ottawa, Ottawa, ON K1H 8M5, Canada; University of Ottawa Brain and Mind Research Institute, University of Ottawa, Ottawa, ON K1H 8M5, Canada. Electronic address: acolavita@ohri.ca.

Abstract

Formation and resolution of multicellular rosettes can drive convergent extension (CE) type cell rearrangements during tissue morphogenesis. Rosette dynamics are regulated by both planar cell polarity (PCP)-dependent and -independent pathways. Here we show that CE is involved in ventral nerve cord (VNC) assembly in Caenorhabditis elegans. We show that a VANG-1/Van Gogh and PRKL-1/Prickle containing PCP pathway and a Slit-independent SAX-3/Robo pathway cooperate to regulate, via rosette intermediaries, the intercalation of post-mitotic neuronal cell bodies during VNC formation. We show that VANG-1 and SAX-3 are localized to contracting edges and rosette foci and act to specify edge contraction during rosette formation and to mediate timely rosette resolution. Simultaneous loss of both pathways severely curtails CE resulting in a shortened, anteriorly displaced distribution of VNC neurons at hatching. Our results establish rosette-based CE as an evolutionarily conserved mechanism of nerve cord morphogenesis and reveal a role for SAX-3/Robo in this process.

KEYWORDS:

convergent-extension; planar cell polarity; sax-3/Robo; vang-1/Van Gogh

PMID:
28441532
PMCID:
PMC5469364
DOI:
10.1016/j.devcel.2017.03.024
[Indexed for MEDLINE]
Free PMC Article

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