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Nutrients. 2017 Apr 25;9(5). pii: E424. doi: 10.3390/nu9050424.

A Study of the Differential Effects of Eicosapentaenoic Acid (EPA) and Docosahexaenoic Acid (DHA) on Gene Expression Profiles of Stimulated Thp-1 Macrophages.

Author information

1
Institute of Nutrition and Functional Foods (INAF), Pavillon des Services, 2440 Hochelaga Blvd, Laval University, Québec City, QC G1V 0A6, Canada. allamndoulbenedicte@gmail.com.
2
Institute of Nutrition and Functional Foods (INAF), Pavillon des Services, 2440 Hochelaga Blvd, Laval University, Québec City, QC G1V 0A6, Canada. Frederic.Guenard@fsaa.ulaval.ca.
3
Laboratory of Molecular Pharmacology, CHU de Québec Research Center, Québec City, QC G1V 0A6, Canada. Olivier.barbier@crchul.ulaval.ca.
4
Institute of Nutrition and Functional Foods (INAF), Pavillon des Services, 2440 Hochelaga Blvd, Laval University, Québec City, QC G1V 0A6, Canada. marie-claude.vohl@fsaa.ulaval.ca.

Abstract

Background: An appropriate intake of omega-3 (n-3) fatty acids (FAs) such as eicosapentaenoic and docosahexaenoic acid (EPA/DHA) from marine sources is known to have anti-inflammatory effects. However, molecular mechanisms underlying their beneficial effects on health are not fully understood. The aim of the present study was to characterize gene expression profiles of THP-1 macrophages, incubated in either EPA or DHA and stimulated with lipopolysaccharide (LPS), a pro-inflammatory agent. Methods: THP-1 macrophages were incubated into 10, 50 and 75 µM of EPA or DHA for 24 h, and 100 nM of LPS was added to the culture media for 18 h. Total mRNA was extracted and gene expression examined by microarray analysis using Illumina Human HT-12 expression beadchips (Illumina). Results: Pathway analysis revealed that EPA and DHA regulate genes involved in cell cycle regulation, apoptosis, immune response and inflammation, oxidative stress and cancer pathways in a differential and dose-dependent manner. Conclusions: EPA and DHA appear to exert differential effects on gene expression in THP-1 macrophages. Specific effects of n-3 FAs on gene expression levels are also dose-dependent.

KEYWORDS:

gene expression; inflammation; mRNA; microarrays; omega-3 fatty acids

PMID:
28441337
PMCID:
PMC5452154
DOI:
10.3390/nu9050424
[Indexed for MEDLINE]
Free PMC Article

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