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Methods Mol Biol. 2017;1580:297-302. doi: 10.1007/978-1-4939-6866-4_20.

Label-Free Direct Detection of MiRNAs with Poly-Silicon Nanowire Biosensors.

Author information

1
Shanghai Integrated Circuit Research & Development Center, 177 Bibo Road, Shanghai, China.
2
School of Life Science, East China Normal University, Shanghai, China.
3
Shanghai Integrated Circuit Research & Development Center, 177 Bibo Road, Shanghai, China. bjiang@icrd.com.cn.

Abstract

The diagnostic and prognostic value of microRNAs (miRNAs) in diseases becomes promising. Owing to fast response and high sensitivity, silicon nanowire (SiNW) biosensor has been considered a potential tool for miRNAs detection. Here, we describe a booming method to detect miRNAs with poly-silicon nanowire biosensors. Standard and real miRNA samples are applied in this study. The results show a limitation of 1 fM in the detection of standard miRNA sample with our poly-nanowire devices. Meanwhile, one-base mismatched sequence could be distinguished. Furthermore, these poly-SiNW arrays can detect snRNA U6 in total RNA samples extracted from HepG2 cells with a detection limitation of 0.2 μg/mL.

KEYWORDS:

SiNW; miRNA

PMID:
28439841
DOI:
10.1007/978-1-4939-6866-4_20
[Indexed for MEDLINE]

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