Format

Send to

Choose Destination
J Microbiol Methods. 2017 Aug;139:12-14. doi: 10.1016/j.mimet.2017.04.008. Epub 2017 Apr 22.

Convenient PCR method for variable-number tandem-repeat typing of Mycobacterium tuberculosis clinical isolates.

Author information

1
Department of Microbiology, Yamagata Prefectural Institute of Public Health, 1-6-6 Toka-machi, Yamagata-shi, Yamagata 990-0031, Japan. Electronic address: setoj@pref.yamagata.jp.
2
Department of International Health, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
3
Department of Microbiology, Yamagata Prefectural Institute of Public Health, 1-6-6 Toka-machi, Yamagata-shi, Yamagata 990-0031, Japan.
4
Department of Mycobacterium Reference and Research, The Research Institute of Tuberculosis, Japan Anti-tuberculosis Association, 3-1-24 Matsuyama, Kiyose, Tokyo 204-8533, Japan.

Abstract

Variable-number tandem-repeat typing for Mycobacterium tuberculosis clinical isolates contributes to evidence-based tuberculosis control. However, cumbersome PCR procedures for the typing have disturbed routine analyses. We proposed a convenient PCR method for the typing using a PCR master mix that provides rapidity and long-term stability of the frozen PCR cocktail.

KEYWORDS:

Frozen PCR cocktail; High labor effectiveness; Human error reduction; Rapidity; SapphireAmp Fast Master Mix

PMID:
28438643
DOI:
10.1016/j.mimet.2017.04.008
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center