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Cryobiology. 2017 Jun;76:154-157. doi: 10.1016/j.cryobiol.2017.04.005. Epub 2017 Apr 21.

First successful vitrification of salmonid ovarian tissue.

Author information

1
Department of Aquaculture, Szent István University, Páter Károly u. 1, H-2100 Gödöllő, Hungary. Electronic address: Lujic.Jelena@mkk.szie.hu.
2
Department of Aquaculture, Szent István University, Páter Károly u. 1, H-2100 Gödöllő, Hungary; Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovica 2, 21000 Novi Sad, Serbia. Electronic address: zor.marinovic@gmail.com.
3
Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, Sl-1230 Domžale, Slovenia. Electronic address: Simona.Susnik@bf.uni-lj.si.
4
Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Groblje 3, Sl-1230 Domžale, Slovenia. Electronic address: Ida.Djurdjevic@bf.uni-lj.si.
5
Department of Aquaculture, Szent István University, Páter Károly u. 1, H-2100 Gödöllő, Hungary. Electronic address: Kasa.Eszter@mkk.szie.hu.
6
Department of Aquaculture, Szent István University, Páter Károly u. 1, H-2100 Gödöllő, Hungary. Electronic address: Urbanyi.Bela@mkk.szie.hu.
7
Department of Aquaculture, Szent István University, Páter Károly u. 1, H-2100 Gödöllő, Hungary. Electronic address: Horvath.Akos@mkk.szie.hu.

Abstract

Due to a lack of cryopreservation protocols for fish eggs and embryos, alternative techniques which will enable storage of female genetic resources are crucial for future development of reproduction management in conservation biology and aquaculture. Experiments were conducted to develop an optimal vitrification protocol for cryopreservation of brown trout Salmo trutta juvenile ovarian tissue. Needle immersed vitrification (NIV) method was used where ovaries were pinned on an acupuncture needle, passaged through equilibration and vitrification solutions containing different combinations and concentrations of methanol (MeOH), propylene glycol (PG) and dimethyl sulfoxide (Me2SO) and subsequently plunged into liquid nitrogen. Vitrification solutions containing equal cryoprotectant concentrations (3M Me2SO and 3M PG) yielded the highest oogonia survival rates (up to 40%) and qualitatively and quantitatively unaltered perinucleolar follicles. The method developed for brown trout could be applied to the conservation of female genetic resources of other salmonid species, including endangered and endemic species or populations.

KEYWORDS:

Brown trout; Cryopreservation; Needle immersed vitrification; Oogonia; Salmo trutta

PMID:
28438562
DOI:
10.1016/j.cryobiol.2017.04.005
[Indexed for MEDLINE]

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