Format

Send to

Choose Destination
Phytochemistry. 2017 Aug;140:1-15. doi: 10.1016/j.phytochem.2017.04.014. Epub 2017 Apr 21.

C21 steroidal glycosides from Cynanchum auriculatum and their neuroprotective effects against H2O2-induced damage in PC12 cells.

Author information

1
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China.
2
State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Taipa, Macau 999078, China.
3
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming, 650500, China. Electronic address: mizhangkmust@126.com.

Abstract

Twenty-three undescribed previously C21 steroidal glycosides, named saccatols D-K and cynsaccatols I-W, together with six known analogs were isolated from the roots of Cynanchum auriculatum Royle ex Wight (Apocynaceae). Their structures were elucidated by a variety of spectroscopic techniques, as well as HRESIMS and GC method. All isolated compounds were tested their neuroprotective effects on the model of H2O2-induced damage to PC12 cells. Except saccatols D-F, J, cynsaccatol V and wilfoside A, other twenty-three isolates produced significantly activities against H2O2-induced cell damage (P < 0.001). Furthermore, cynsaccatols I, N, O and S can obvious inhibition of damaged PC12 cell apoptosis at their dosages of 1 μM by Annexin V-FITC/PI double staining assay with flow cytometry.

KEYWORDS:

Apocynaceae; Apoptosis; C(21) steroidal glycosides; Cynanchum auriculatum; Neuroprotecton; PC12

PMID:
28437621
DOI:
10.1016/j.phytochem.2017.04.014
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center