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Int J Nanomedicine. 2017 Apr 10;12:2933-2939. doi: 10.2147/IJN.S130160. eCollection 2017.

Efficacy of silk fibroin-nano silver against Staphylococcus aureus biofilms in a rabbit model of sinusitis.

Author information

1
Department of Otolaryngology-Head and Neck Surgery, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou.
2
Department of Otolaryngology-Head and Neck Surgery, Huashan Hospital, Fudan University.
3
State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Laboratory of Advanced Materials, Fudan University, Shanghai, China.

Abstract

BACKGROUND:

Staphylococcus aureus biofilms contribute significantly to the recalcitrant nature of chronic rhinosinusitis. In previous studies, it has been shown that silk fibroin-nano silver solution can eliminate S. aureus biofilms in vitro, which suggests a potential role of this novel agent in the treatment of biofilm-associated diseases, such as sinusitis.

OBJECTIVE:

The aim of this study was to investigate the efficacy of silk fibroin-nano silver solution as a topical anti-biofilm agent in a rabbit model of sinusitis.

METHODS:

Biofilm-associated sinusitis models were established in 24 New Zealand White rabbits by gelatin sponge placement and S. aureus inoculation through a hole drilled into the anterolateral wall of the right maxillary sinus. After 4 weeks, indwelling catheters were placed into the maxillary sinus. Different concentrations of silk fibroin-nano silver solution or normal saline were irrigated slowly into the maxillary sinus via the indwelling catheters. After 7 days of irrigation, the rabbits were sacrificed. The sinus mucosa was harvested and examined for biofilm biomass as well as morphological integrity of the epithelium by scanning electron microscopy.

RESULTS:

Silk fibroin-nano silver solution was found to be most effective in reducing the biomass of the S. aureus biofilms at a concentration of 384 mg/L, followed by the concentration of 153.6 mg/L, when compared with saline. After treatment with 384 mg/L silk fibroin-nano silver solution, the biofilms were completely eliminated and the injured epithelium was almost restored with regenerated cilia on the surface.

CONCLUSION:

Silk fibroin-nano silver solution was found to be an effective topical agent against S. aureus biofilms in the rabbit model of sinusitis, and its effect was concentration-dependent.

KEYWORDS:

animal; biomass; chronic rhinosinusitis; maxillary sinus; nasal irrigation; scanning electron microscopy

PMID:
28435269
PMCID:
PMC5391841
DOI:
10.2147/IJN.S130160
[Indexed for MEDLINE]
Free PMC Article

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